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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
优利科(上海)生命科学有限公司
- 库存:
100
- 靶点:
详询
- 级别:
科研
- 目录编号:
/
- 克隆性:
多克隆
- 抗原来源:
详见说明书
- 保质期:
1年
- 抗体英文名:
Anti-Insulin Receptor/CD220
- 抗体名:
Insulin Receptor/CD220抗体
- 标记物:
详询
- 宿主:
兔
- 适应物种:
/
- 免疫原:
见产品详情
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
见产品详情
- 保存条件:
-20℃
- 浓度:
1mg/ml
- 规格:
详询
| 英文名称 Anti-Insulin Receptor/CD220 |
| 中文名称 胰岛素受体抗体 |
| 别 名 CD 220; CD220; CD220 antigen; HHF 5; HHF5; INSR; IR; INSR_HUMAN; Insulin receptor subunit beta. |
| 浓 度 1mg/1ml |
| 规 格 0.1ml/100μg 0.2ml/200μg |
| 抗体来源 Rabbit |
| 克隆类型 polyclonal |
| 交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep |
| 产品类型 一抗 |
研究领域 细胞生物 神经生物学 信号转导 细胞凋亡 细胞膜受体 内分泌病 细胞表面分子 糖蛋白 ![]() |
| 蛋白分子量 predicted molecular weight: 165kDa |
| 性 状 Lyophilized or Liquid |
| 免 疫 原 KLH conjugated synthetic peptide derived from human IR N-terminus |
| 亚 型 IgG |
| 纯化方法 affinity purified by Protein A |
| 储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
| 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| (石蜡切片需做抗原修复) |
| not yet tested in other applications. |
| optimal dilutions/concentrations should be determined by the end user. |
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. ![]() |
| Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 The insulin receptor is a heterotetrameric membrane glycoprotein with tyrosine-protein kinase activity, consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit possesses a single transmembrane domain, whereas the alpha subunit is completely extracellular. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Binding of insulin to the insulin receptor stimulates its association with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K) which leads to glucose uptake. Two transcript variants encoding different isoforms have been found for this gene produced by alternative splicing. |
| Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
| 胰岛素受体是一个四聚体,由两个α亚基和两个β亚基通过二硫键连接。两个α亚基位于细胞质膜的外侧,其上有胰岛素的结合位点;两个β亚基是跨膜蛋白,起信号转导作用。无胰岛素结 合时,受体的酪氨酸蛋白激酶没有活性。当胰岛素与受体的α亚基结合并改变了β亚基的构型后,酪氨酸蛋白激酶才被激活,激活后可催化两个反应: |
| ①使四聚体复合物中β亚基特异位点的酪氨酸残基磷酸化,这种过程称为自我磷酸化(autophosphorylation); |
| ②将胰岛素受体底物(insulin receptor substrate,IRSs)上具有重要作用的十几个酪氨酸残基磷酸化,磷酸化的IRSs能够结合并激活下游效应物。 |
(isoform CRA-c)胰岛素受体是一种跨膜蛋白,含两个α亚基(135kda)和两个β亚基(分子量:97kda)。胰岛素受体是胰岛素得传感装置,可在细胞内和细胞膜循环。![]() |
| Anti-Insulin(1-51 protein) 胰岛素1-51蛋白抗体 |
| Anti-Integrin α3β1 整合素α3β1抗体 |
| Anti-Integrin α4β7/LPAM-1 (Lymphocyte Peyer’s patch Adhesion Molecule 1/Integrin alpha4,beta7) 整合素α4β7抗体 |
| anti-Integrin avb3 整合素avb3抗体 |
| Anti-Integrin αE2/CD103 整合素αE2抗体 |
| Anti-IQGAP1 支架蛋白抗体 |
| Anti-IRS P53 (Insulin receptor suYTtrate P53) 胰岛素受体底物p53蛋白抗体 |
| Anti-IRS-1 胰岛素受体底物-1抗体 |
| Anti-IRS-2 胰岛素受体底物-2抗体 |
| Anti-IRS-3 胰岛素受体底物-3抗体 |
| Anti-IRS-4 胰岛素受体底物-4抗体 |
| Anti-ISR(Insulin Receptor) 胰岛素受体抗体 |
| Anti-ISR-α (Insulin Receptor-α) 胰岛素受体-α抗体 |
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文献和实验人乙酰胆碱脂酶受体抗体(AchR-Ab)酶联免疫分析(ELISA)
人 乙酰胆碱脂酶受体抗体( AchR-Ab ) 酶联免疫分析(ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中乙酰胆碱脂酶受体抗体(AchR-Ab )的 含量。 实验原理: 本试剂盒应用双抗原夹心法测定标本中人乙酰胆碱脂酶受体抗体( AchR-Ab ) 水平。 用纯化的抗原包被微孔板,制成固相抗原,往包被单抗的微孔中依次加入抗乙酰胆碱脂酶受体抗体
baolingbaby 各位前辈,鄙人现NCBI菜鸟一个,现急需胰岛素基因序列以及它各个内含子和外显子的序列,如果能帮我附上图文解说的查询方法更感激不尽,我也尝试从头开始学NCBI但时间太紧了,我还得分析其他数据,各位拜托了,谢谢。我的邮箱是baolingbaby@163.com,结果太大的话可以发到我邮箱里。再次谢谢了 yuandong 或许对你有用http://liucheng.name/1048/ 另外,摸索一下
佚名 胰岛素是含有51个氨基酸的小分子蛋白质,分子量为6000,胰岛素分子有靠两个二硫键结合的A链(21个氨基酸)与B链(30个氨基酸),如果二硫键被打开则失去活性(图11-21)。B细胞先合成一个大分子的前胰岛素原,以后加工成八十六肽的胰岛素原,再经水解成为胰岛素与连接肽(C肽)。 图11-21 人胰岛素的化学结构 胰岛素与C肽共同













