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Anti-Insulin Receptor/CD220
Insulin Receptor/CD220抗体
详询
兔
/
见产品详情
IgG
Lyophilized or Liquid
见产品详情
-20℃
1mg/ml
详询
英文名称 Anti-Insulin Receptor/CD220 |
中文名称 胰岛素受体抗体 |
别 名 CD 220; CD220; CD220 antigen; HHF 5; HHF5; INSR; IR; INSR_HUMAN; Insulin receptor subunit beta. |
浓 度 1mg/1ml |
规 格 0.1ml/100μg 0.2ml/200μg |
抗体来源 Rabbit |
克隆类型 polyclonal |
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep |
产品类型 一抗 |
研究领域 细胞生物 神经生物学 信号转导 细胞凋亡 细胞膜受体 内分泌病 细胞表面分子 糖蛋白 |
蛋白分子量 predicted molecular weight: 165kDa |
性 状 Lyophilized or Liquid |
免 疫 原 KLH conjugated synthetic peptide derived from human IR N-terminus |
亚 型 IgG |
纯化方法 affinity purified by Protein A |
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide |
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
(石蜡切片需做抗原修复) |
not yet tested in other applications. |
optimal dilutions/concentrations should be determined by the end user. |
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 The insulin receptor is a heterotetrameric membrane glycoprotein with tyrosine-protein kinase activity, consisting of disulfide-linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit possesses a single transmembrane domain, whereas the alpha subunit is completely extracellular. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Binding of insulin to the insulin receptor stimulates its association with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K) which leads to glucose uptake. Two transcript variants encoding different isoforms have been found for this gene produced by alternative splicing. |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
胰岛素受体是一个四聚体,由两个α亚基和两个β亚基通过二硫键连接。两个α亚基位于细胞质膜的外侧,其上有胰岛素的结合位点;两个β亚基是跨膜蛋白,起信号转导作用。无胰岛素结 合时,受体的酪氨酸蛋白激酶没有活性。当胰岛素与受体的α亚基结合并改变了β亚基的构型后,酪氨酸蛋白激酶才被激活,激活后可催化两个反应: |
①使四聚体复合物中β亚基特异位点的酪氨酸残基磷酸化,这种过程称为自我磷酸化(autophosphorylation); |
②将胰岛素受体底物(insulin receptor substrate,IRSs)上具有重要作用的十几个酪氨酸残基磷酸化,磷酸化的IRSs能够结合并激活下游效应物。 |
(isoform CRA-c)胰岛素受体是一种跨膜蛋白,含两个α亚基(135kda)和两个β亚基(分子量:97kda)。胰岛素受体是胰岛素得传感装置,可在细胞内和细胞膜循环。 |
Anti-Insulin(1-51 protein) 胰岛素1-51蛋白抗体 |
Anti-Integrin α3β1 整合素α3β1抗体 |
Anti-Integrin α4β7/LPAM-1 (Lymphocyte Peyer’s patch Adhesion Molecule 1/Integrin alpha4,beta7) 整合素α4β7抗体 |
anti-Integrin avb3 整合素avb3抗体 |
Anti-Integrin αE2/CD103 整合素αE2抗体 |
Anti-IQGAP1 支架蛋白抗体 |
Anti-IRS P53 (Insulin receptor suYTtrate P53) 胰岛素受体底物p53蛋白抗体 |
Anti-IRS-1 胰岛素受体底物-1抗体 |
Anti-IRS-2 胰岛素受体底物-2抗体 |
Anti-IRS-3 胰岛素受体底物-3抗体 |
Anti-IRS-4 胰岛素受体底物-4抗体 |
Anti-ISR(Insulin Receptor) 胰岛素受体抗体 |
Anti-ISR-α (Insulin Receptor-α) 胰岛素受体-α抗体 |
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