相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
优利科(上海)生命科学有限公司
- 库存:
100
- 靶点:
详询
- 级别:
科研
- 目录编号:
/
- 克隆性:
多克隆
- 抗原来源:
详见说明书
- 保质期:
1年
- 抗体英文名:
Anti-IGFALS/ALS
- 抗体名:
IGFALS/ALS抗体
- 标记物:
详询
- 宿主:
兔
- 适应物种:
/
- 免疫原:
KLH conjugated synthetic peptide derived from human IGFALS/ALS
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
- 保存条件:
-20℃
- 浓度:
1mg/ml
- 规格:
详询
| 英文名称 Anti-IGFALS/ALS |
| 中文名称 胰岛素样生长因子酸不稳定亚基ALS抗体 |
| 别 名 Albs; IGFALS; Insulin like growth factor binding protein acid labile subunit; Insulin like growth factor binding protein complex acid labile chain; MGC150140; MGC150141; KIAA4111; mKIAA4111; ALS_HUMAN. |
| 浓 度 1mg/1ml |
| 规 格 0.2ml/200μg |
| 抗体来源 Rabbit |
| 克隆类型 polyclonal |
| 交叉反应 Human, Mouse, Rat |
| 产品类型 一抗 |
研究领域 心血管 信号转导 生长因子和激素 ![]() |
| 蛋白分子量 predicted molecular weight: 63kDa |
| 性 状 Lyophilized or Liquid |
| 免 疫 原 KLH conjugated synthetic peptide derived from human IGFALS/ALS |
| 亚 型 IgG |
| 纯化方法 affinity purified by Protein A |
| 储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| 产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 |
| (石蜡切片需做抗原修复) |
| not yet tested in other applications. |
| optimal dilutions/concentrations should be determined by the end user. |
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. ![]() |
| Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 The Insulin-like growth factor binding proteins, or IGFBPs, are a family of seven proteins that have co-evolved with the IGFs. IGFBPs serve as shuttle molecules for both IGF-I and IGF-II and confer a level of regulation to the IGF signaling system by influencing the bio-availability, concentration and distribution of IGFs in the extracellular environment. In human circulation, the IGF-binging protein complex requires ALS (IGFBP acid-labile subunit), an extracellular protein involved in receptor-ligand binding and cell adhesion. ALS, detected primarily in plasma, is involved in protein-protein interactions that result in the formation of protein complexes. |
| Function : Involved in protein-protein interactions that result inprotein complexes, receptor-ligand binding or cell adhesion. |
| Subunit : Forms a ternary complex of about 140 to 150 kDa withIGF-I or IGF-II and IGFBP-3. |
| Subcellular Location : Secreted; extracellular space. |
| Tissue Specificity : Plasma. |
| Similarity : Contains 19 LRR (leucine-rich) repeats. |
| Contains 1 LRRCT domain. |
| Contains 1 LRRNT domain. |
Database links : UniProtKB/Swiss-Prot: P35858.1![]() |
| N-coR1 (Nuclear receptor co-repressor 1) 核受体辅助抑制因子(抗原) |
| Nestin 巢蛋白(神经上皮干细胞蛋白)(抗原) |
| Nestin 巢蛋白(神经上皮干细胞蛋白)(抗原) |
| neurofasciu-155 神经束蛋白-155 |
| NGF-β 神经生长因子-β(多肽片断抗原) |
| NGF-R(p75NTR) 神经生长因子受体(抗原) |
| NIK,NF-kappaB-Inducing Kinase NFkB诱导的激酶(抗原) |
| ATF1 (Activating Transcription Factor1) 活化复制因子1 |
| NKB (Neurokinins B) 神经激肽B(抗原) |
| NKR ; Neurokin B receptor (Neuromedin K receptor; NK-4 receptor) 神经激肽-B受体(抗原) |
| Nociceptin 孤菲肽(痛敏肽)(抗原) |
| Nociceptin receptor 孤菲肽受体(痛敏肽受体)(抗原) |
| Nogo-A 轴索过度生长抑制因子-A(抗原) |
| Nogo-66/NEP1-40 Nogo-66肽(1-40)多肽 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人胰岛素样生长因子结合蛋白 1(IGFBP-1)酶联免疫分析(ELISA)
) 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 胰岛素样生长因子结合蛋白 1(IGFBP-1) ,再与 HRP 标记的胰岛素样生长因子结合蛋白 1(IGFBP-1) 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 胰岛素样生长因子结合蛋白 1(IGFBP-1) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品
人胰岛素样生长因子-1(IGF-1)酶联免疫分析(ELISA)
板,往微孔中依次加入 胰岛素样生长因子 - Ⅰ (IGF- Ⅰ ) ,再与 HRP 标记的 IGF- Ⅰ 抗体结合,形成免疫复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 胰岛素样生长因子 - Ⅰ (IGF- Ⅰ ) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中 胰岛素样生长因子 - Ⅰ (IGF- Ⅰ ) 抗原浓度。 试剂盒组成
饥饿的 HeLa 和 NIH 3T3 癌细胞系获得的裂解物中的磷酸化蛋白特异性。图示:从复杂生物样品中富集高纯度磷蛋白。用 theThermo Scientific Pierce 磷酸化蛋白富集试剂盒进行 Weste blot 分析,根据试剂盒说明书制备细胞裂解液,富集磷酸化蛋白。利用识别生长因子信号传导相关关键调控蛋白的磷酸化特异性抗体实现蛋白检测。细胞色素 C (pI 9.6) 和 p15Ink4b (pI 5.5) 作为非磷酸化蛋白非特异性结合的阴性对照。FT = 流穿馏分,W = 合并洗脱馏分,E













