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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Liquid
- 保存条件:
负20℃
- 标记物:
生物素标记
- 适应物种:
Mus musculus (Mouse)
- 保质期:
Aliquot and store at -20ºC for 12 months
- 应用范围:
WB; IHC; ICC.
- 浓度:
200μg-1mg/ml
- 抗体英文名:
Biotin-Linked Polyclonal Antibody to Collagen Type III Alpha 1 (COL3a1)
- 规格:
20-100μl
以下是产品说明范例,由于产品众多,Biotin标记Ⅲ型胶原α1(COL3a1)多克隆抗体详细说明书请向客服免费索取,谢谢!
[ PROPERTIES ]
Source: Antibody labeling
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label: HRP
Original Antibody: Rab(P)12288
Traits: Liquid
Concentration: 0.5mg/ml
UOM: 200μl
Cross Reactivity: Mouse; Rat
Applications: WB; IHC; ICC.
[ APPLICATIONS ]
Western blotting: 0.5-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,
50% glycerol.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
公司所有产品仅供科研,不作临床诊断、医药或者其他用途。
[ PROPERTIES ]
Source: Antibody labeling
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label: HRP
Original Antibody: Rab(P)12288
Traits: Liquid
Concentration: 0.5mg/ml
UOM: 200μl
Cross Reactivity: Mouse; Rat
Applications: WB; IHC; ICC.
[ APPLICATIONS ]
Western blotting: 0.5-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,
50% glycerol.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
公司所有产品仅供科研,不作临床诊断、医药或者其他用途。
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文献和实验相关实验
变性,需在4℃进行,同时用pH8.0缓冲液稀释抗血清,以减少蛋白浓度过高而发生共沉淀。铵盐的溶解度不随温度变化而明显改变,0℃和25℃仅差3%,而钠盐则相差5倍,因此常在低温沉淀时用铵盐,室温沉淀用钠盐。铵盐对抗体的标记反应(如FITC和biotin标记时)有一定的干扰作用。 盐析法只能部分纯化抗体,更高纯度的抗体制剂可用层析法制备。IgM五聚体相对分子质量达9.7×10,比血清中任何其他蛋白都大,用分子筛层析很容易将其纯化。IgG在PH8.0时带负电荷,能与DEAE纤维素上的阳离子结合,因此可用
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Biotin标记Ⅲ型胶原α1(COL3a1)多克隆抗体
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