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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
优利科(上海)生命科学有限公司
- 库存:
120
- 靶点:
详询
- 级别:
科研
- 目录编号:
/
- 克隆性:
多克隆
- 抗原来源:
详见说明书
- 保质期:
1年
- 抗体英文名:
Anti-IPMK
- 抗体名:
IPMK抗体
- 标记物:
详询
- 宿主:
Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep
- 适应物种:
/
- 免疫原:
KLH conjugated synthetic peptide derived from human IPMK
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 保存条件:
-20℃
- 浓度:
1mg/ml
- 规格:
详询
| 英文名称 Anti-IPMK |
| 中文名称 多磷酸肌醇激酶IPMK抗体 |
| 别 名 6-tetrakisphosphate 5-kinase; Inositol 1; Inositol 1,3,4,6 tetrakisphosphate 5 kinase; Inositol polyphosphate multikinase; Ipmk; IPMK_HUMAN |
| 浓 度 1mg/1ml |
| 规 格 0.2ml/200μg |
| 抗体来源 Rabbit |
| 克隆类型 polyclonal |
| 交叉反应 Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep |
| 产品类型 一抗 |
| 研究领域 细胞生物 发育生物学 信号转导 干细胞 转录调节因子 激酶和磷酸酶 表观遗传学 |
| 蛋白分子量 predicted molecular weight: 47kDa |
| 性 状 Lyophilized or Liquid |
免 疫 原 KLH conjugated synthetic peptide derived from human IPMK ![]() |
| 亚 型 IgG |
| 纯化方法 affinity purified by Protein A |
| 储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| 产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| (石蜡切片需做抗原修复) |
| not yet tested in other applications. |
| optimal dilutions/concentrations should be determined by the end user. |
| 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 Inositol polyphosphate multikinase (IPMK) belongs to the inositol phosphokinase (IPK) family and is characterized as having a broad substrate specificity. However, IPMK displays a preference for inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). IPMK is ubiquitously expressed with the highest expression in skeletal muscle, liver, placenta, lung, peripheral blood leukocytes, kidney, spleen and colon. IPMK is localized to the nucleus, where it may play a role in the regulation of calcium release from intracellular stores and has been implicated as a drug target for cancer therapies. The gene encoding IPMK maps to human chromosome 10, which contains over 800 genes. Notably, disorders linked to genes on chromosome 10 include Cowden syndrome, Cockayne syndrome and Tetrahydrobiopterin deficiency. |
| Function : Inositol phosphate kinase with a broad substrate specificity. Has a preference for inositol-1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). |
Subcellular Location : Nucleus.![]() |
| Tissue Specificity : Ubiquitous, with the highest expression in skeletal muscle, liver, placenta, lung, peripheral blood leukocytes, kidney, spleen and colon. |
| Similarity : Belongs to the inositol phosphokinase (IPK) family. |
| Database links : UniProtKB/Swiss-Prot: Q8NFU5.1 |
| IGF-II( Insulin-like Growth Factor-II ) 胰岛素样生长因子-II(抗原) |
| TP-5(Thymopentin) 胸腺五肽 |
| Galanin 神经节肽(抗原) |
| GLP-2(Glucagon-like peptide-2) 胰高血糖素样肽-2蛋白 |
| ADNP (NAP) (Activity-dependent Neuroprotective protein ) 活性依赖的神经保护肽(抗原) |
| AACT, Alpha-1-Antichymotrypsin α-1抗胰糜蛋白酶(抗原) |
| ACTH (Adrenorticotrophin hormons)(1-39) 促肾上腺皮质激素(1-39)(抗原) |
| ADM/AM Protein (Adrenomedullin) 肾上腺髓质素(1-52) |
| ACTH (Adrenorticotrophin hormons)(18-39) 促肾上腺皮质激素(18-39)(抗原) |
| CCK8 (Cholecystokinin-8 Peptide) 胆囊收缩素/缩胆囊素(八肽) |
| SP (SuYTtance P) P物质(抗原) |
| Ghrelin 脑肠肽(一种新的生长激素释放肽)多肽抗原 |
| Gastrin(human) 胃泌素/蛙皮素(多肽人源) |
| myelin P0 prothein( peripheral myelin prothein Zero;MPZ;MPP) 外周髓鞘蛋白0(P0蛋白)多肽抗原 |
| Insulin(1-51 protein) 胰岛素(1-51 蛋白) |
| Tachyplesin I(Tachyplesin-1 precursor ) 抗菌肽 |
| PAR-2 AP (Protease-activated receptors-2 Activating polypeptide) 蛋白酶激活受体-2激活肽 |
| PAR-2 OP(Protease-activated receptors-2 Activating Orange-peptide) 蛋白酶激活受体-2桔抗 |
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文献和实验inside-out mode)。这种方法的细胞体积越大,破坏部位离被吸附膜片越远或破坏孔越小,均可导致细胞质因子外流变慢。 3. 膜外面向外模式(outside-out mode) 如果从上述的全细胞模式将膜片微电极向上提起可得到切割分离的膜片,由于它的细胞膜外侧面面对膜片微电极腔内液,膜外面自然封闭而对外,所以这个模式被称为膜外面向外模式(outside-out mode)。此构型多用于研究细胞膜外侧受体控制的离子通道,研究腺苷酸环化酶、多磷酸磷脂酰肌醇激酶、蛋白激酶C等活动性变化,以及细胞膜上信使物质
(酶)的活性有很大的变化,PI3K/AKT通路就是一个典型的例子。这也是信号传导研究 需要用到很多磷酸化蛋白的抗体的重要原因。 聪仔 顶!! 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴 师兄微信号:shixiongcoming
了使用放射性同位素时的危险品和废物处理要求。许多磷酸化特异抗体十分灵敏,可轻松检测常规样品(如10-30 µg细胞提取物)中的磷酸化蛋白。由于测得的磷酸化蛋白水平可能随处理或凝胶上样误差而变化,研究人员常常利用一个抗体来检测同源蛋白的总水平(而不考虑磷酸化状态),以确定磷酸化组分相对于总组分的比例,并充当上样内对照。化学发光和显色法都很常用,而分子量marker常用来提供蛋白分子量的信息。详细的Western blot实验步骤请看www.RnDSystems.com













