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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100⁺瓶
- 英文名:
D-Lys-(D-Lys)n-D-Lys·xHBr
- 保质期:
1年
- 供应商:
上海睿安生物13611631389
- 保存条件:
−20°C
- 规格:
500mg/瓶
现货Sigma货号P0899-500mg多聚-D-赖氨酸氢溴suān盐(规格包装500mg/瓶)(目录价¥11522.33元/瓶)13611631389上海睿安生物

应用:
多聚-D-赖氨酸氢溴suān盐被用于以下研究:
●动物细胞培养
●大鼠皮层神经元培养
●细胞培养和siRNA处理
●原代细胞培养和成人神经元网络模型
●皮质星形胶质细胞培养
●免疫荧光染色[将HDF细胞接种到多聚-D-赖氨酸(P0899,Sigma-Aldrich)包被的玻璃盖玻片上]
●NPC收集和培养条件

聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5-1.0ml的该产品0.1mg/mL溶液用作细胞培养的基质对25cm²进行涂覆。较低分子量的该产品粘度相对较低,但较高分子量的形式则可提供每分子更多的附着位点。
包装:10 mg in glass bottle;50mg,100mg,500mg in poly bottle;1g in poly bottle。
生化/生理作用:该产品是一种细胞的非特异性附着因子,可用于通过增强细胞膜的带负电离子与培养表面之间的静电相互作用而促进细胞与固体基质的粘附。当被细胞培养表面吸收后,聚-D-赖氨酸可增加带正电的细胞结合位点数量。
组分:聚-D-赖氨酸是一种带正电的氨基酸聚合物,其中每个赖氨酸残基约有一个HBr。氢溴suān盐可使聚-D-赖氨酸呈可溶于水的结晶形式。在β结构中可能会发现少量的产物,因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意:无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
分析说明:
本品的分子量为70000-150000。为了除去HBr,将本品溶解在中性缓冲液中并透析除去盐。通常,本品用作粘附因子,将50ml无菌组织培养级水加至5mg多聚赖氨酸中,无菌条件下,每25cm²表面包被1ml。包被5分钟,然后吸去溶液并充分清表面。晾干2小时,然后加入细胞和培养基。



同行评审论文(部分文献)
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文献和实验该产品被引用文献
现货Sigma货号P0899-500mg多聚-D-赖氨酸氢溴suān盐(规格包装500mg/瓶)(目录价¥11522.33元/瓶)13611631389上海睿安生物

应用:
多聚-D-赖氨酸氢溴suān盐被用于以下研究:
●动物细胞培养
●大鼠皮层神经元培养
●细胞培养和siRNA处理
●原代细胞培养和成人神经元网络模型
●皮质星形胶质细胞培养
●免疫荧光染色[将HDF细胞接种到多聚-D-赖氨酸(P0899,Sigma-Aldrich)包被的玻璃盖玻片上]
●NPC收集和培养条件

聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5-1.0ml的该产品0.1mg/mL溶液用作细胞培养的基质对25cm²进行涂覆。较低分子量的该产品粘度相对较低,但较高分子量的形式则可提供每分子更多的附着位点。
包装:10 mg in glass bottle;50mg,100mg,500mg in poly bottle;1g in poly bottle。
生化/生理作用:该产品是一种细胞的非特异性附着因子,可用于通过增强细胞膜的带负电离子与培养表面之间的静电相互作用而促进细胞与固体基质的粘附。当被细胞培养表面吸收后,聚-D-赖氨酸可增加带正电的细胞结合位点数量。
组分:聚-D-赖氨酸是一种带正电的氨基酸聚合物,其中每个赖氨酸残基约有一个HBr。氢溴suān盐可使聚-D-赖氨酸呈可溶于水的结晶形式。在β结构中可能会发现少量的产物,因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意:无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
分析说明:
本品的分子量为70000-150000。为了除去HBr,将本品溶解在中性缓冲液中并透析除去盐。通常,本品用作粘附因子,将50ml无菌组织培养级水加至5mg多聚赖氨酸中,无菌条件下,每25cm²表面包被1ml。包被5分钟,然后吸去溶液并充分清表面。晾干2小时,然后加入细胞和培养基。



同行评审论文(部分文献)
BMI-1026 treatment can induce SAHF formation by activation of Erk1/2.
Hyun-Joo Seo et al.
BMB reports, 41(7), 523-528 (2008-08-07)
BMI-1026 is a synthetic aminopyrimidine compound that targets cyclin dependent kinases (cdks) and was initially designed as a potential anticancer drug. Even though it has been well documented that BMI-1026 is a potent cdk inhibitor, little is known about the
Tetanus toxin and botulinum toxin a utilize unique mechanisms to enter neurons of the central nervous system.
Faith C Blum et al.
Infection and immunity, 80(5), 1662-1669 (2012-03-07)
Botulinum neurotoxins (BoNTs) and tetanus neurotoxin (TeNT) are the most toxic proteins for humans. While BoNTs cause flaccid paralysis, TeNT causes spastic paralysis. Characterized BoNT serotypes enter neurons upon binding dual receptors, a ganglioside and a neuron-specific protein, either synaptic
Biomimicking Fiber Scaffold as an Effective In Vitro and In Vivo MicroRNA Screening Platform for Directing Tissue Regeneration.
Na Zhang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(9), 1800808-1800808 (2019-05-09)
MicroRNAs effectively modulate protein expression and cellular response. Unfortunately, the lack of robust nonviral delivery platforms has limited the therapeutic application of microRNAs. Additionally, there is a shortage of drug-screening platforms that are directly translatable from in vitro to in
Myc increases self-renewal in neural progenitor cells through Miz-1.
Laura Kerosuo et al.
Journal of cell science, 121(Pt 23), 3941-3950 (2008-11-13)
The mechanisms underlying the decision of a stem or progenitor cell to either self-renew or differentiate are incompletely understood. To address the role of Myc in this process, we expressed different forms of the proto-oncogene Myc in multipotent neural progenitor
Morphological and physiological changes in mature in vitro neuronal networks towards exposure to short-, middle- or long-term simulated microgravity.
Giuseppe Pani et al.
PloS one, 8(9), e73857-e73857 (2013-09-26)
One of the objectives of the current international space programmes is to investigate the possible effects of the space environment on the crew health. The aim of this work was to assess the particular effects of simulated microgravity on mature
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