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2719736-43-1
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DNA-PK-IN-5
CAS No. : 2719736-43-1
MCE 国际站：DNA-PK-IN-5
产品活性：DNA-PK-IN-5 是一种有效的 DNA-PK 抑制剂。DNA-PK-IN-5 抑制 DNA-PKcs 活性，从而大大降低肿瘤 DNA 修复并诱导细胞进入凋亡程序。DNA-PK-IN-5 增强肿瘤组织对放疗的敏感性，克服耐药问题，增强对多种实体瘤和血液肿瘤的抑制作用 (摘自专利 WO2021204111A1，化合物 2)。
研究领域：Cell Cycle/DNA Damage | PI3K/Akt/mTOR
作用靶点：DNA-PK
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
aqueous DNA into new tube. SOLUTIONS Digestion buffer: 1X PCR Buffer with 0.5% Tween20 / 0.4mg/ml pk stored in aliquots at -20 C. PK: Stored as 20mg/ml aliquots at -20 C; can be refrozen a few times. Methyl Green Staining
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
and and pipette aqueous DNA into new tube. SOLUTIONS Digestion buffer: 1X PCR Buffer with 0.5% Tween20 / 0.4mg/ml pk stored in aliquots at -20 ℃. PK: Stored as 20mg/ml aliquots at -20 ℃; can be refrozen a few times. Methyl Green Staining Methyl Green is used
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
. We also include a "mock" samples which contains 1X dialysis buffer instead of chromatin (no antibody and mock are critical to control for nonspecific interactions and DNA contamination of Ip and wash solutions....the final output of this experiment is analyzed
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