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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Goat Anti-Mouse IgG2a, Human ads-TRITC
- CAS号：
Goat Anti-Mouse IgG2a, Human ads-TRITC
- 保质期：
2年
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
1.0mg
山羊抗小鼠 IgG2a，人ads-TRITC、Goat Anti-Mouse IgG2a, Human ads-TRITC详细如下：
山羊抗小鼠 IgG 2a -TRITC 抗体，对人类蛋白质的反应性极小，可用于免疫组织化学/免疫细胞化学分析。
同型：山羊IgG
同型对照：山羊IgG-TRITC
特异性：与小鼠 IgG 2a 的重链反应
来源：来自用小鼠 IgG 2a 超免疫的山羊的混合抗血清
交叉吸附：小鼠 IgG 1、IgG 2b、IgG 3、IgM 和 IgA；人免疫球蛋白和混合血清；可能与其他物种的免疫球蛋白发生反应
净化方法：共价连接琼脂糖 的小鼠 IgG 2a 的亲和层析
共轭：TRITC（罗丹明）
缓冲液配方：含有 < 0.1% 叠氮hua钠的磷酸盐缓冲盐水
克隆性：多克隆
浓度：1.0 毫克/毫升
体积：1.0 毫升
推荐存储：2-8℃；避免暴露在光线下
应用
相关格式的质量测试应用包括 -
ELISA 1-9
FLISA
流式细胞仪11-13
相关格式的其他参考应用包括 -
ELISpot 3,10
免疫组织化学-冷冻切片2,14,15
免疫组织化学-石蜡切片16,17
免疫细胞化学4,18 -20
电子显微镜14
Western Blot 1,21-23
表面等离子共振24
纯化25
Citations
1. Martin RM, Brady JL, Lew AM. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J Immunol Methods. 1998;212:187-92. (ELISA, WB)
2. Richards HB, Satoh M, Jennette JC, Croker BP, Yoshida H, Reeves WH. Interferon-γ is required for lupus nephritis in mice treated with the hydrocarbon oil pristane. Kidney Int. 2001;60:2173-80. (ELISA, IHC-FS)
3. Cox RJ, Mykkeltvedt E, Robertson J, Haaheim LR. Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication. Scand J Immunol. 2002;55:14-23. (ELISA, ELISPOT)
4. Swiecki MK, Lisanby MW, Shu F, Turnbough CL Jr, Kearney JF. Monoclonal antibodies for Bacillus anthracis spore detection and functional analyses of spore germination and outgrowth. J Immunol. 2006;176:6076-84. (ELISA, ICC)
5. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell mediated model of oral tolerance. Mucosal Immunol. 2009;2:427-38. (ELISA)
6. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
7. Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79. (ELISA)
8. Dabral N, Jain-Gupta N, Seleem MN, Sriranganathan N, Vemulapalli R. Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide. Front Cell Infect Microbiol. 2015;5:54. (ELISA)
9. Dabaghian M, Latifi AM, Tebianian M, Dabaghian F, Ebrahimi SM. A truncated C-terminal fragment of Mycobacterium tuberculosis HSP70 enhances cell-mediated immune response and longevity of the total IgG to influenza A virus M2e protein in mice. Antiviral Res. 2015;120:23-31. (ELISA)
10. Teichmann LL, Schenten D, Medzhitov R, Kashgarian M, Shlomchik MJ. Signals via the adaptor MyD88 in B cells and DCs make distinct and synergistic contributions to immune activation and tissue damage in lupus. Immunity. 2013;38:528-40. (ELISPOT)
11. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
12. Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, et al. Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β. Nature. 2012;484:514-8. (FC)
13. Sutton KM, Hu T, Wu Z, Siklodi B, Vervelde L, Kaiser P. The functions of the avian receptor activator of NF-κB ligand (RANKL) and its receptors, RANK and osteoprotegerin, are evolutionarily conserved. Dev Comp Immunol. 2015;51:170-84. (FC)
14. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
15. Poosti F, Bansal R, Yazdani S, Prakash J, Post E, Klok P, et al. Selective delivery of IFN-γ to renal interstitial myofibroblasts: a novel strategy for the treatment of renal fibrosis. FASEB J. 2015;29:1029-42. (IHC-FS)
16. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (IHC-PS)
17. Kreijtz JH, Wiersma LC, De Gruyter HL, Vogelzang-van Trierum SE, van Amerongen G, Stittelaar KJ, et al. A single immunization with modified vaccinia virus Ankara-based influenza virus H7 vaccine affords protection in the influenza A(H7N9) pneumonia ferret model. J Infect Dis. 2015;211:791-800. (IHC-PS)
18. Albrecht B, Woisetschläger M, Robertson MW. Export of the high affinity IgE receptor from the endoplasmic reticulum depends on a glycosylation-mediated quality control mechanism. J Immunol. 2000;165:5686-94. (ICC)
19. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
20. Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)
21. Santoro L, Reboul A, Kerblat I, Drouet C, Colomb MG. Monoclonal IgG as antigens: reduction is an early intracellular event of their processing by antigen-presenting cells. Int Immunol. 1996;8:211-9. (WB)
22. Attramadal CG, Halstensen TS, Dhakal HP, Ulekleiv CH, Boysen ME, Nesland JM, et al. High nuclear SOX2 expression is associated with radiotherapy response in small (T1/T2) oral squamous cell carcinoma. J Oral Pathol Med. 2015;44:515-22. (WB)
23. Konitsiotis AD, Jovanović B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate EW, et al. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein. J Biol Chem. 2015;290:3293-307. (WB)
24. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (Surface Plasmon Resonance)
25. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (Purification)
山羊抗小鼠 IgG 2a -TRITC 抗体，对人类蛋白质的反应性极小，可用于免疫组织化学/免疫细胞化学分析。
同型：山羊IgG
同型对照：山羊IgG-TRITC
特异性：与小鼠 IgG 2a 的重链反应
来源：来自用小鼠 IgG 2a 超免疫的山羊的混合抗血清
交叉吸附：小鼠 IgG 1、IgG 2b、IgG 3、IgM 和 IgA；人免疫球蛋白和混合血清；可能与其他物种的免疫球蛋白发生反应
净化方法：共价连接琼脂糖 的小鼠 IgG 2a 的亲和层析
共轭：TRITC（罗丹明）
缓冲液配方：含有 < 0.1% 叠氮hua钠的磷酸盐缓冲盐水
克隆性：多克隆
浓度：1.0 毫克/毫升
体积：1.0 毫升
推荐存储：2-8℃；避免暴露在光线下
应用
相关格式的质量测试应用包括 -
ELISA 1-9
FLISA
流式细胞仪11-13
相关格式的其他参考应用包括 -
ELISpot 3,10
免疫组织化学-冷冻切片2,14,15
免疫组织化学-石蜡切片16,17
免疫细胞化学4,18 -20
电子显微镜14
Western Blot 1,21-23
表面等离子共振24
纯化25
Citations
1. Martin RM, Brady JL, Lew AM. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J Immunol Methods. 1998;212:187-92. (ELISA, WB)
2. Richards HB, Satoh M, Jennette JC, Croker BP, Yoshida H, Reeves WH. Interferon-γ is required for lupus nephritis in mice treated with the hydrocarbon oil pristane. Kidney Int. 2001;60:2173-80. (ELISA, IHC-FS)
3. Cox RJ, Mykkeltvedt E, Robertson J, Haaheim LR. Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication. Scand J Immunol. 2002;55:14-23. (ELISA, ELISPOT)
4. Swiecki MK, Lisanby MW, Shu F, Turnbough CL Jr, Kearney JF. Monoclonal antibodies for Bacillus anthracis spore detection and functional analyses of spore germination and outgrowth. J Immunol. 2006;176:6076-84. (ELISA, ICC)
5. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell mediated model of oral tolerance. Mucosal Immunol. 2009;2:427-38. (ELISA)
6. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
7. Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79. (ELISA)
8. Dabral N, Jain-Gupta N, Seleem MN, Sriranganathan N, Vemulapalli R. Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide. Front Cell Infect Microbiol. 2015;5:54. (ELISA)
9. Dabaghian M, Latifi AM, Tebianian M, Dabaghian F, Ebrahimi SM. A truncated C-terminal fragment of Mycobacterium tuberculosis HSP70 enhances cell-mediated immune response and longevity of the total IgG to influenza A virus M2e protein in mice. Antiviral Res. 2015;120:23-31. (ELISA)
10. Teichmann LL, Schenten D, Medzhitov R, Kashgarian M, Shlomchik MJ. Signals via the adaptor MyD88 in B cells and DCs make distinct and synergistic contributions to immune activation and tissue damage in lupus. Immunity. 2013;38:528-40. (ELISPOT)
11. Davis ME, Maxwell CV, Erf GF, Brown DC, Wistuba TJ. Dietary supplementation with phosphorylated mannans improves growth response and modulates immune function of weanling pigs. J Anim Sci. 2004;82:1882-91. (FC)
12. Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, et al. Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β. Nature. 2012;484:514-8. (FC)
13. Sutton KM, Hu T, Wu Z, Siklodi B, Vervelde L, Kaiser P. The functions of the avian receptor activator of NF-κB ligand (RANKL) and its receptors, RANK and osteoprotegerin, are evolutionarily conserved. Dev Comp Immunol. 2015;51:170-84. (FC)
14. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
15. Poosti F, Bansal R, Yazdani S, Prakash J, Post E, Klok P, et al. Selective delivery of IFN-γ to renal interstitial myofibroblasts: a novel strategy for the treatment of renal fibrosis. FASEB J. 2015;29:1029-42. (IHC-FS)
16. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (IHC-PS)
17. Kreijtz JH, Wiersma LC, De Gruyter HL, Vogelzang-van Trierum SE, van Amerongen G, Stittelaar KJ, et al. A single immunization with modified vaccinia virus Ankara-based influenza virus H7 vaccine affords protection in the influenza A(H7N9) pneumonia ferret model. J Infect Dis. 2015;211:791-800. (IHC-PS)
18. Albrecht B, Woisetschläger M, Robertson MW. Export of the high affinity IgE receptor from the endoplasmic reticulum depends on a glycosylation-mediated quality control mechanism. J Immunol. 2000;165:5686-94. (ICC)
19. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
20. Mia MM, Bank RA. Paracrine factors of human amniotic fluid-derived mesenchymal stem cells show strong anti-fibrotic properties by inhibiting myofibroblast differentiation and collagen synthesis. J Stem Cell Res Ther. 2015;5:5. (ICC)
21. Santoro L, Reboul A, Kerblat I, Drouet C, Colomb MG. Monoclonal IgG as antigens: reduction is an early intracellular event of their processing by antigen-presenting cells. Int Immunol. 1996;8:211-9. (WB)
22. Attramadal CG, Halstensen TS, Dhakal HP, Ulekleiv CH, Boysen ME, Nesland JM, et al. High nuclear SOX2 expression is associated with radiotherapy response in small (T1/T2) oral squamous cell carcinoma. J Oral Pathol Med. 2015;44:515-22. (WB)
23. Konitsiotis AD, Jovanović B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate EW, et al. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein. J Biol Chem. 2015;290:3293-307. (WB)
24. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (Surface Plasmon Resonance)
25. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (Purification)
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山羊抗小鼠 IgG2a，人ads-TRITC
￥3200
