山羊抗鼠 Lambda-FITC、Goat Anti-Mouse Lambda-FITC详细如下: 山羊抗小鼠 Lambda-FITC 抗体,用于流式细胞术、免疫组织化学/免疫细胞化学和蛋白质印迹分析。 同型:山羊IgG 同型对照:山羊IgG-FITC 特异性:与小鼠 λ 轻链反应 来源:来自用小鼠λ轻链超免疫山羊的混合抗血清 交叉吸附:小鼠κ轻链;可能与来自其他物种的 λ 轻链发生反应 净化方法:与琼脂糖共价连接的小鼠 λ 轻链的亲和层析 共轭:FITC(荧光素) 缓冲液配方:含有 < 0.1% 叠氮hua钠的磷酸盐缓冲盐水 克隆性:多克隆 浓度:1.0 毫克/毫升 体积:1.0 毫升 推荐存储:2-8℃;避免暴露在光线下 应用 相关格式的质量测试应用包括 - ELISA 1-6,23 FLISA 流式细胞术3,5,7-13,24 相关格式的其他参考应用包括 - ELISpot 6,7 免疫组化-冷冻切片2,13-16 免疫组化-石蜡切片17,23 免疫细胞化学1,4,18 蛋白质 印迹19,20,25 免疫沉淀21,22 刺激23,24 分离25 Citations 1. Gollahon KA, Hagman J, Brinster RL, Storb U. Ig λ-producing B cells do not show feedback inhibition of gene rearrangement. J Immunol. 1988;141:2771-80. (ELISA, ICC) 2. Mandik-Nayak L, Nayak S, Sokol C, Eaton-Bassiri A, Madaio MP, Caton AJ, et al. The origin of anti-nuclear antibodies in bcl-2 transgenic mice. Int Immunol. 2000;12:353-64. (ELISA, IHC-FS) 3. Shivtiel S, Leider N, Melamed D. Receptor editing in CD45-deficient immature B cells. Eur J Immunol. 2002;32:2264-73. (ELISA, FC) 4. Ghumra A, Semblat J, McIntosh RS, Raza A, Rasmussen IB, Braathen R, et al. Identification of residues in the Cμ4 domain of polymeric IgM essential for interaction with Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). J Immunol. 2008;181:1988-2000. (ELISA, ICC) 5. Liu X, Shen S, Manser T. Influence of B cell antigen receptor expression level on pathways of B cell tolerance induction. J Immunol. 2009;182:398-407. (ELISA, FC) 6. Willems KN. Regulation of humoral immunity by Pim kinases [dissertation]. Worcester (USA): University of Massachusetts; 2011. (ELISA, ELISPOT) 7. Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19. (ELISPOT, FC) 8. Adachi T, Wakabayashi C, Nakayama T, Yakura H, Tsubata T. CD72 negatively regulates signaling through the antigen receptor of B cells. J Immunol. 2000;164:1223-9. (FC) 9. Su Y, Flemming A, Wossning T, Hobeika E, Reth M, Jumaa H. Identification of a pre-BCR lacking surrogate light chain. J Exp Med. 2003;198:1699-706. (FC) 10. Hayashi K, Nojima T, Goitsuka R, Kitamura D. Impaired receptor editing in the primary B cell repertoire of BASH-deficient mice. J Immunol. 2004;173:5980-8. (FC) 11. Vettermann C, Herrmann K, Albert C, Roth E, Bösl MR, Jäck H. A unique role for the λ5 nonimmunoglobulin tail in early B lymphocyte development. J Immunol. 2008;181:3232-42. (FC) 12. Heizmann B, Kastner P, Chan S. Ikaros is absolutely required for pre-B cell differentiation by attenuating IL-7 signals. J Exp Med. 2013;210:2823-32. (FC) 13. Aslam M, Kishi Y, Tsubata T. Excess CD40L does not rescue anti-DNA B cells from clonal anergy. F1000Res. 2013;2:218. (FC, IHC-FS) 14. Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, et al. Disruption of the Bcl6 gene results in an impaired germinal center formation. J Exp Med. 1997;186:439-48. (IHC-FS) 15. Mandik-Nayak L, Huang G, Sheehan KC, Erikson J, Chaplin DD. Signaling through TNF receptor p55 in TNF-α-deficient mice alters the CXCL13/CCL19/CCL21 ratio in the spleen and induces maturation and migration of anergic B cells into the B cell follicle. J Immunol. 2001;167:1920-8. (IHC-FS, ICC) 16. Rouaud P, Saintamand A, Saad F, Carrion C, Lecardeur S, Cogné M, et al. Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region. J Exp Med. 2014;211:975-85. (IHC-FS) 17. Weinstein JS, Nacionales DC, Lee PY, Kelly-Scumpia KM, Yan X, Scumpia PO, et al. Colocalization of antigen-specific B and T cells within ectopic lymphoid tissue following immunization with exogenous antigen. J Immunol. 2008;181:3259-67. (IHC-PS) 18. Ghumra A, Shi J, Mcintosh RS, Rasmussen IB, Braathen R, Johansen F, et al. Structural requirements for the interaction of human IgM and IgA with the human Fcα/μ receptor. Eur J Immunol. 2009;39:1147-56. (ICC) 19. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in CH1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB) 20. Sato M, Adachi T, Tsubata T. Augmentation of signaling through BCR containing IgE but not that containing IgA due to lack of CD22-mediated signal regulation. J Immunol. 2007;178:2901-7. (WB) 21. Shen Y, Meunier L, Hendershot LM. Identification and characterization of a novel endoplasmic reticulum (ER) DnaJ homologue, which stimulates ATPase activity of BiP in vitro and is induced by ER stress. J Biol Chem. 2002;277:15947-56. (IP) 22. Behnke J, Hendershot LM. The large Hsp70 Grp170 binds to unfolded protein substrates in vivo with a regulation distinct from conventional Hsp70s. J Biol Chem. 2014;289:2899-907. (IP) 23. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (ELISA, IHC-FS, Stim) 24. Übelhart R, Bach MP, Eschbach C, Wossning T, Reth M, Jumaa H. N-linked glycosylation selectively regulates autonomous precursor BCR function. Nat Immunol. 2010;11:759-65. (FC, Stim) 25. Yurovsky VV, Kelsoe G. Pairing of VH gene families with the λ1 light chain: evidence for a non-stochastic association. Eur J Immunol. 1993;23:1975-9. (WB, Sep)