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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源生物
- 库存:
Mouse IgG-AP
- 靶点:
Mouse IgG-AP
- 级别:
Mouse IgG-AP
- 目录编号:
Mouse IgG-AP
- 克隆性:
多克隆
- 抗原来源:
小鼠 IgG
- 保质期:
一年
- 抗体英文名:
Mouse IgG-AP
- 抗体名:
小鼠 IgG-AP
- 标记物:
齐源生物
- 宿主:
Mouse IgG-AP
- 适应物种:
Mouse IgG-AP
- 免疫原:
Mouse IgG-AP
- 亚型:
Mouse IgG-AP
- 形态:
Mouse IgG-AP
- 应用范围:
Mouse IgG-AP
- 保存条件:
2-8℃
- 浓度:
Mouse IgG-AP
- 规格:
1.0mL
小鼠 IgG-AP 用作免疫组织化学/免疫细胞化学测定中的对照。
同型:小鼠 IgG
来源:正常小鼠血清
共轭:AP(碱性磷酸酶)
缓冲液配方:50 mM Tris/1 mM MgCl 2 /50% 甘油,含 < 0.1% 叠氮hua钠,pH 8.0
克隆性:多克隆
浓度:特定批次
体积:1.0 毫升
推荐存储:2-8°C
应用
相关格式的质量测试应用包括 -
流式细胞术7-10
ELISA 1-6
FLISA
相关格式的其他参考应用包括 -
免疫组织化学 - 石蜡切片11
免疫细胞化学12
蛋白质印迹13,14
免疫沉淀15,16
染色质免疫沉淀17,18
封闭19, 20
体外对照22-25
体内对照2,9,21
Citations
1. Kuklin N, Daheshia M, Karem K, Manickan E, Rouse BT. Induction of mucosal immunity against herpes simplex virus by plasmid DNA immunization. J Virol. 1997;71:3138-45. (ELISA)
2. Cupples AL. Addition of an anti-DNA antibody enhances plasmid DNA vaccination against herpes simplex virus [thesis]. Knoxville (USA): University of Tennessee; 2004. (ELISA, in vivo control)
3. Genestier L, Taillardet M, Mondiere P, Gheit H, Bella C, Defrance T. TLR agonists selectively promote terminal plasma cell differentiation of B cell subsets specialized in thymus-independent responses. J Immunol. 2007;178:7779-86. (ELISA)
4. Wang Y, Diamond B. B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice. J Clin Invest. 2008;118:2896-907. (ELISA)
5. Szymczak WA, Davis MJ, Lundy SK, Dufaud C, Olszewski M, Pirofski L. X-linked immunodeficient mice exhibit enhanced susceptibility to Cryptococcus neoformans infection. Mbio. 2013;4(4): e00265-13. (ELISA)
6. Mann JF, McKay PF, Fiserova A, Klein K, Cope A, Rogers P, et al. Enhanced immunogenicity of an HIV-1 DNA vaccine delivered with electroporation via combined intramuscular and intradermal routes. J Virol. 2014;88:6959-69. (ELISA)
7. Nataf S, Davoust N, Ames RS, Barnum SR. Human T cells express the C5a receptor and are chemoattracted to C5a. J Immunol. 1999;162:4018-23. (FC)
8. Butler JE, Moore MB, Presnell SR, Chan H, Chalupny NJ, Lutz CT. Proteasome regulation of ULBP1 transcription. J Immunol. 2009;182:6600-9. (FC)
9. O'Neill AF, Dearling JL, Wang Y, Tupper T, Sun Y, Aster JC, et al. Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody. Clin Cancer Res. 2014;20:678-87. (FC, in vivo control)
10. Silvescu CI, Sackstein R. G-CSF induces membrane expression of a myeloperoxidase glycovariant that operates as an E-selectin ligand on human myeloid cells. Proc Natl Acad Sci USA. 2014;111:10696-701. (FC)
11. Howell MD, Kim BE, Gao P, Grant AV, Boguniewicz M, DeBenedetto A, et al. Cytokine modulation of atopic dermatitis filaggrin skin expression. J Allergy Clin Immunol. 2007;120:150-5. (IHC-PS)
12. Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
13. Lintomen L, Elsas MI, Maximiano ES, de Paula Neto HA, Joseph D, Vargaftig BB, et al. Allergenic sensitization prevents upregulation of haemopoiesis by cyclo-oxygenase inhibitors in mice. Br J Pharmacol. 2002;135:1315-23. (WB)
14. Xu J, Cui G, Esmailian F, Plunkett M, Marelli D, Ardehali A, et al. Atrial extracellular matrix remodeling and the maintenance of atrial fibrillation. Circulation. 2004;109:363-8. (WB)
15. Inagaki T, Iwaski S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77. (IP)
16. Li G, Nguyen CC, Ryckman BJ, Britt WJ, Kamil JP. A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism. Proc Natl Acad Sci USA. 2015;112:4471-6. (IP)
17. Breslin MB, Wang H, Pierce A, Aucoin R, Lan MS. Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1. FEBS Lett. 2007;581:949-54. (ChIP)
18. Tao Y, Sharif N, Zeng B, Cai Y, Guo Y. Lateral ventricle injection of orexin-A ameliorates central precocious puberty in rat via inhibiting the expression of MEG3. Int J Clin Exp Pathol. 2015;8:12564-70. (ChIP)
19. Bühring H, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007;1106:262-71. (Block)
20. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (Block)
21. Arumugam T, Deng D, Bover L, Wang H, Logsdon CD, Ramachandran V. New blocking antibodies against novel AGR2-C4.4A pathway reduce growth and metastasis of pancreatic tumors and increase survival in mice. Mol Cancer Ther. 2015;14:941-51. (In vivo control)
22. Nakajima H, Samaridis J, Angman L, Colonna M. Cutting edge: Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor γ-chain. J Immunol. 1999;162:5-8. (In vitro control)
23. Milbauer LC, Enenstein JA, Roney M, Solovey A, Bodempudi V, Nichols TC, et al. Blood outgrowth endothelial cell migration and trapping in vivo: a window into gene therapy. Transl Res. 2009;153:179-89. (In vitro control)
24. Gibb DR, Shikh ME, Kang D, Rowe WJ, El Sayed R, Cichy J, et al. ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. J Exp Med. 2010;207:623-35. (In vitro control)
25. Xu G, Wen X, Hong Y, Du H, Zhang X, Song J, et al. An anti-transferrin receptor antibody enhanced the growth inhibitory effects of chemotherapeutic drugs on human glioma cells. Int Immunopharmacol. 2011;11:1844-9. (In vitro control)
26. Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (Size exclusion chromatography control). (Size exclusion chromatography control)
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文献和实验superman211 拟通过WB进行AP-1的相关研究,在选择一抗时遇到如下困惑,望了解的战友予以解答。关于AP-1的出c-jun的一抗有多种,其中主要分为磷酸化一抗、非磷酸化一抗两大类。每一大类又包含若干,不知如何选择,它们之间有何区别?实验目的为研究某细胞AP-1的表达情况,及其蛋白磷酸化和DNA结合活性等。 superman211 自顶,望解答,谢谢! ziyunfei ampk细胞
人抗磷脂抗体IgG(APL-IgG)酶联免疫分析(ELISA)
人抗磷脂抗体IgG(APL-IgG) 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中抗磷脂抗体IgG(APL-IgG) 的含量。 实验原理: 本试剂盒应用双抗原夹心法测定标本中人抗磷脂抗体 IgG(APL-IgG) 水平。用纯化的抗磷脂抗体 IgG(APL-IgG) 抗原包被微孔板,制成固相抗原,往包被抗原的微孔中依次加入抗磷脂抗体
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