相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Mouse IgG2b-PE
- CAS号:
Mouse IgG2b-PE
- 保质期:
1年
- 供应商:
齐源生物
- 保存条件:
2-8℃
- 规格:
100tests
小鼠 IgG 2b -PE 用作流式细胞仪检测的对照。
克隆:A-1
同型:小鼠 IgG 2b κ
特异性:鸡 IgA
共轭:PE(R-藻红蛋白)
缓冲液配方:含有 < 0.1% 叠氮hua钠和稳定剂的磷酸盐缓冲盐水
克隆性:单克隆
浓度:特定批次
体积:1.0 毫升
推荐存储:2-8℃;避免暴露在光线下;不要冻结
应用
流式细胞术 – 质量检测7-11
ELISA – 质量检测1-6
FLISA – 质量检测
免疫组织化学-冷冻切片 – 文献报道11,12
免疫组织化学-石蜡切片14
体外对照 – 文献报道6
Citations
1. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)
2. Roopenian DC, Christianson GJ, Sproule TJ, Brown AC, Akilesh S, Jung N, et al. The MHC class I-like IgG receptor controls perinatal IgG transport, IgG homeostasis, and fate of IgG-Fc-coupled drugs. J Immunol. 2003;170:3528-33. (ELISA)
3. Maier M, Seabrook TJ, Lemere CA. Modulation of the humoral and cellular immune response in Aβ immunotherapy by the adjuvants monophosphoryl lipid A (MPL), cholera toxin B subunit (CTB) and E. coli enterotoxin LT(R192G). Vaccine. 2005;23:5149-59. (ELISA)
4. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA)
5. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
6. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
7. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC, in vitro control)
8. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC)
9. Charerntantanakul W, Platt R, Roth JA. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61. (FC)
10. Ghimire TR, Benson RA, Garside P, Brewer JM. Alum increases antigen uptake, reduces antigen degradation and sustains antigen presentation by DCs in vitro. Immunol Lett. 2012;147:55-62. (FC)
11. Yoon S, Zhang X, Lee IY, Spencer N, Vo P, Choi YS. CD9 is a novel marker for plasma cell precursors in human germinal centers. Biochem Biophys Res Commun. 2013;431:41-6. (FC, IHC-FS)
12. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (IHC-FS)
13. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control)
14. SouthernBiotech published data (IHC-PS)
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验小鼠腹水及单细胞悬液制备流程 配制 6% 淀粉肉汤。 6% 淀粉肉汤配制方法:牛肉膏 0.3g、蛋白胨 1.0g、氯化钠 0.5g、蒸馏水 100mL,上述材料混合加热后加入可溶性淀粉 6.0g;溶解后,121℃ 高压灭菌 15~20 min,EP 管分装封口,将肉汤放置于 4℃ 保存。 小鼠腹腔注射 1mL 6% 淀粉肉汤(注意避开肠管和内脏),刺激 60~72h。 颈椎脱臼法处死小鼠,用 75% 酒精浸泡小鼠 5min。 将小鼠置于解剖板中,固定四肢,剪开皮肤,充分暴露腹膜。 用眼
,无菌复溶 9. 淋巴细胞M密度梯度分离液(Accurate Chemical and Scientific),室温 10. RPMI-10完全培养基 11. FITC标记的抗CD3抗体和PE标记的PK136抗体(PharMingen) 12. 15ml和50ml带盖聚丙烯锥底离心管 13. Beckman CS-6R离心机(或同类离心机) 14. 血清学吸管 实验方法: 1. 处死小鼠,无菌取脾脏。 2. 制备脾脏
0:00 富集和检测小鼠分泌IL-17的活T细胞0 1:30 内容简介90 2:10 配制试剂130 3:07 刺激脾细胞187 5:19 用捕获试剂标记细胞319 7:36 用IL-17检测抗体(生物素)和抗生物素PE标记细胞456 9:50 FACS分析590 11:40 结论700 本视频来源于网络,如有异议请联系我们,我们将在5个工作日内作出处理。
技术资料暂无技术资料 索取技术资料






![58569-55-4*[Met5]-脑啡肽](https://img1.dxycdn.com/2022/0701/373/6940918926551567553-14.jpg!wh200)



