小鼠 IgG1-CY5 (15H6)、Mouse IgG1-CY5详细如下: 小鼠 IgG 1 -CY5 用作流式细胞术和免疫组织化学/免疫细胞化学测定的对照。 克隆:15H6 同型:小鼠 IgG 1 κ 特异性:T-2霉菌毒素 共轭:CY5(花青 5) 缓冲液配方:含有 < 0.1% 叠氮hua钠的磷酸盐缓冲盐水 克隆性:单克隆 浓度:特定批次 体积:1.0 毫升 推荐存储:2-8℃;避免暴露在光线下 应用 流式细胞术 – 质量测试5-10,12,14 ELISA – 质量测试1-4 FLISA – 质量测试 免疫组织化学-冷冻切片 – 文献报道11,12 免疫组织化学-石蜡切片 – 文献报道13,23 免疫细胞化学 – 文献报道14,15 Western Blot——文献报道16,17 Blocking——文献报道8 体外对照——文献报道5,7,12,14,18体内对照——文献报道19-21 Multiplex——文献报道24 Citations 1. Fritz JH, Rojas OL, Simard N, McCarthy DD, Hapfelmeirer S, Rubino S, et al. Acquisition of a multifunctional IgA+ plasma cell phenotype in the gut. Nature. 2012;481:199-203. (ELISA) 2. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA) 3. DiLillo DJ, Tan GS, Palese P, Ravetch JV. Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo. Nat Med. 2014;20:143-51. (ELISA) 4. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA) 5. Charerntantanakul W, Platt R, Roth JA. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61. (FC, in vitro control) 6. Zurney J, Howard KE, Sherry B. Basal expression levels of IFNAR and Jak-STAT components are determinants of cell-type-specific differences in cardiac antiviral responses. J Virol. 2007;81:13668-80. (FC) 7. Chua W, Kim S, Myers N, Huang S, Yu L, Fremont DH, et al. Endogenous MHC-related protein 1 is transiently expressed on the plasma membrane in a conformation that activates mucosal-associated invariant T cells. J Immunol. 2011;186:4744-50. (FC, in vitro control) 8. Sinka L, Biasch K, Khazaal I, Péault B, Tavian M. Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo. Blood. 2012;119:3712-23. (FC, Block) 9. Troyer RM, Thompson J, Elder JH, VandeWoude S. Accessory genes confer a high replication rate to virulent feline immunodeficiency virus. J Virol. 2013;87:7940-51. (FC) 10. Weckbach LT, Gola A, Winkelmann M, Jakob SM, Groesser L, Borgolte J, et al. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via β2 integrins (CD11/CD18). Blood. 2014;123:1887-96. (FC) 11. Kiekens RC, Thepen T, Bihari IC, Knol EF, Van De Winkel JG, Bruijnzeel-Koomen CA. Expression of Fc receptors for IgG during acute and chronic cutaneous inflammation in atopic dermatitis. Br J Dermatol. 2000;142:1106-13. (IHC-FS) 12. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC, IHC-FS, in vitro control) 13. Roberts S, Kosanke S, Dunn ST, Jankelow D, Duran CM, Cunningham MW. Pathogenic mechanisms in rheumatic carditis: focus on valvular endothelium. J Infect Dis. 2001;183:507-11. (IHC-PS) 14. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC, ICC, in vitro control) 15. Sleeman KE, Kendrick H, Ashworth A, Isacke CM, Smalley MJ. CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Res. 2006;8:R7. (ICC) 16. Howell MD, Kim BE, Gao P, Grant AV, Boguniewicz M, DeBenedetto A, et al. Cytokine modulation of atopic dermatitis filaggrin skin expression. J Allergy Clin Immunol. 2007;120:150-5. (WB) 17. Lucas CR, Cordero-Nieves HM, Erbe RS, McAlees JW, Bhatia S, Hodes RJ, Campbell KS, et al. Prohibitins and the cytoplasmic domain of CD86 cooperate to mediate CD86 signaling in B lymphocytes. J Immunol. 2013;190:723-36. (WB) 18. Hsu H, Baldwin CL, Telfer JC. The endocytosis and signaling of the γδ T cell coreceptor WC1 are regulated by a dileucine motif. J Immunol. 2015;194:2399-406. (In vitro control) 19. Gong J, Yang DJ, Kohanim S, Angelo LS, Kurzrock R. Molecular imaging of gefitinib activity in an epidermal growth factor receptor (EGFR)-bearing xenograft model. Cancer Biol Ther. 2009;8:2237-45. (In vivo control) 20. Xie F, Sun D, Schering A, Nakao S, Zandi S, Liu P, et al. Novel molecular imaging approach for subclinical detection of iritis and evaluation of therapeutic success. Am J Pathol. 2010;177:39-48. (In vivo control) 21. Sun D, Nakao S, Xie F, Zandi S, Bagheri A, Rezaei Kanavi M, et al. Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis. FASEB J. 2014;28:3942-51. (In vivo control) 22. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control) 23. French KJ, Coatney RW, Renninger JP, Hu CX, Gales TL, Zhao S, et al. Differences in effects on myocardium and mitochondria by angiogenic inhibitors suggest separate mechanisms of cardiotoxicity. Toxicol Pathol. 2010;38:691-702. (IHC-PS) 24. Hönger G, Hopfer H, Arnold M, Spriewald BM, Schaub S, Amico P. Pretransplant IgG subclasses of donor-specific human leukocyte antigen antibodies and development of antibody-mediated rejection. Transplantation. 2011;92:41-7. (Multiplex)