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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C/-80°C
- 保质期:
12个月
- 英文名:
Recombinant Human E3 ubiquitin-protein ligase ZNRF3(ZNRF3)
- 库存:
4
- 供应商:
上海禾午生物科技有限公司
- 规格:
20ug/100ug/1mg
产品详情
纯度:Greater than 95% as determined by SDS-PAGE.
内毒素:Less than 1.0 EU/ug as determined by LAL method.
生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human ZNRF3 at 5 μg/mL can bind Human RSPO2, the EC50 is 5.091-6.991 ng/mL.
基因名:ZNRF3
Uniprot No.:Q9ULT6
别名:(RING finger protein 203)(RING-type E3 ubiquitin transferase ZNRF3)(Zinc/RING finger protein 3)
种属:Homo sapiens (Human)
蛋白长度:Partial
来源:Mammalian cell
分子量:20.4 kDa
表达区域:56-219aa
蛋白标签:C-terminal 6xHis-tagged
产品提供形式:Lyophilized powder
功能:E3泛素蛋白连接酶,通过介导Wnt受体复合体Frizzled和LRP6的泛素化和降解,起到Wnt信号通路的负调控作用。同时作用于规范和非规范Wnt信号通路。通过抑制Wnt信号通路,在肠干细胞区作为抑癌因子,从而限制肠干细胞区大小。与RSPO2和RNF43一起,构成了支配分支规格的主开关。
更多活性蛋白请浏览上海禾午生物科技有限公司
上海禾午生物科技有限公司 提供重组蛋白生产服务 His、GST、MBP等多种表达方式同时进行,确保获得蛋白
蛋白表达与纯化服务 纯化的蛋白经过ELISA,Western-blot,或者其它生物活性验证 标签切除 测序报告
上海禾午生物科技有限公司是一家生物高新技术企业,产品覆盖分子生物学、细胞生物学、生物学试剂、标准物质,等领域。以研发、销售为核心致力于生命科学产品。已与全国各大科研院校、医院、企业以及国外一些科研机构有良好的合作关系。
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文献和实验Reconstitution of CHIP E3 Ubiquitin Ligase Activity
CHIP, the carboxyl-terminus of Hsp70 interacting protein, is both an E3 ubiquitin ligase and an Hsp70 co-chaperone and is implicated in the degradation of cytosolic quality control and numerous disease substrates. CHIP has been shown
Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
LubX is one such bacterial E3 ubiquitin ligase. We describe here the method to purify soluble LubX protein using GST-tag and Escherichia coli overexpression systems. Using the purified protein together with recombinant ubiquitin, E1, and E2 enzymes
Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
ubiquitin protein ligase activity to wild-type (but not mutant) Parkin proteins. Although the individual components of the proposed Parkin ubiquitin ligase complex in the normal human brain remain to be identified and the E3 ligase effect of Parkin function
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