属性形式liquid质量水平100manufacturer/tradenameNovagen®储存条件OK to freezeavoid repeated freeze/thaw cycles储存温度10-30°C相关类别Whole Cell Lysis Reagents & Enhancers说明一般描述BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.应用BugBuster® Protein Extraction Reagent has been used:to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purificationas a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescencein lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)警告毒性:标准处理(A)法律信息BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, GermanyNOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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