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物料
PVDF membrane
质量水平
400
无菌性
ethylene oxide treated
sterile
特点
hydrophilic
inlet/outlet Female Luer-Lok/Male Luer slip
包装
pk of 100
manufacturer/tradename
Millipore SLGVR13SL
参数
10 mL sample volume
100 mL (Process Volume)
45 °C max. temp.
7 bar max. inlet pressure at 100 psi
高度
21 mm
过滤器直径
13 mm
颜色
natural
基质
MF-Millipore®
孔径
0.22 μm
相关类别
一般描述
Millex® sterile syringe filters are ideal for sterilizing organic solvents, aqueous solutions or air/gas. Many sample preparation methods specify Millex filters because of their unsurpassed quality and consistency. Millex filters not only feature minimal hold-up volume and faster flow rates, but they also withstand higher operating pressures and are chemically compatible with a range of samples and solvents to minimize leaching of extractable impurities. Millex filters are produced in a controlled, automated environment with the highest quality standards. Sterile devices come with a certificate of quality, with select devices marked for medical applications.应用
A 13 mm diameter sterile syringe filter with a 0.22 μm pore size hydrophilic PVDF membrane. Comes in a pack of 100.Sterilization of tissue culture media, protein solutions or aqueous solutionsUltra-Low Protein Binding
特点和优势
Features & Benefits:- Tailored to a variety of applications based on membrane type, housing and validation rating
- Specialized filter units to protect hemodialysis transducers from blood and moisture and for vacuum line protection
- Low extractables and low absorption membranes for minimum sample losses
- Faster flow rates and higher operating pressures for high performance needs
Sterilization of Aqueous Solutions, Organic Solvents and Gases, Filtration of High Value Protein or Small Molecule Components
其他说明
Directions for Use- Organism Retention: Microorganisms
- Mode of Action: Filtering
- Application: Sterile Filtration
- Intended Use: Sterilization of small volumes of aqueous solutions
- Instructions for Use: Please refer to Instructions for Use section of Millex® syringe filter user guide
- Storage Statement: Store in dry location
- Disposal Statement: Dispose of in accordance with applicable federal, state and local regulations.
法律信息
MF-Millipore is a registered trademark of Merck KGaA, Darmstadt, GermanyMillex is a registered trademark of Merck KGaA, Darmstadt, Germany
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文献和实验失活的小牛血清),其中含1ng/ml PMA和500ng/ml娄诺霉素(Sigma, Saint Louis, MO)。加2.104至5.104个细胞到抗体包被的PVDF孔,孵育箱中孵育10-15小时。其它的刺激剂孵育时间可能不同,基于细胞因子 生成细胞的量,按不同情况作最佳选择。 试剂的准备:1. 10ml磷酸缓冲盐(PBS ,10X)以90ml蒸馏水稀释;2. 0.22g脱脂乳用11ml稀释的PBS溶解,最终浓度为2%;3. 0.22gBSA溶解于22ml已稀释的PBS,最终
。但不适合荧光。PVDF膜特别注意的是需要100%甲醇预处理(不超过15秒)再用缓冲液平衡,才能用,而且适用过程中万一干了也要同样程序再处理(不过要真出现这种问题也是自己欠扁,谁要你不小心呢,转膜前处理也就罢了,转膜后再这样处理可能会影响后继的抗体识别呢)。PVDF膜同样分0.45um和0.2um的,后者孔径小,对小分子蛋白有较好的拦截吸附,背景可能会比前者稍高。 pvdf膜生产商: MILLIPORE 特价 常年 现货 IPVH00010 0.45um
-Rad 蛋白来源:脑组织 蛋白名称(可保密): 蛋白分子量:16 KD、27 KD、32 KD、42 KD WB用膜类型、孔径:PVDF 0.22 um 转膜方式(恒压、恒流):半干转 恒流 1 mA/cm2或2 mA/cm
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