1×反应缓冲液[10mM Bis Tris 丙烷-HCl,10mM MgCl2,1mM DTT(pH 7.0储存于25℃)],37℃温育。
热失活:
70℃,20分钟。
参考文献:
Electrochemical DNA sensor based on T-Hg-T pairs and exonuclease III for sensitive detection of Hg2+[J]. Xuan Wang et al. Sensors and Actuators B-Chemical. 2021.
A Sensitive Fluorescence “Turn-Off-On” Biosensor for Poly (ADP-ribose) Polymerase-1 Detection Based on Cationic Conjugated Polymer-MnO2 Nanosheets[J]. ShuangshuangWu et al. Sensors and Actuators B: Chemical. 2018.