艾本德 Eppendorf Research plus 8

Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period

should be shake gently by rotating blood mixer (vortex) Pour 500 µl of blood into a 1.5 ml eppendorf tube and add 1000 µl of red cell lysis buffer. Shake microfuge tube gently (up to homogenizing), then spin for 2 minutes at 7000 rpm. Discard

Chromatin IP (CHIP assay)

once with 20 ml Pbs transfer to 2 ml Eppendorf tube, wash again with Pbs and freeze cells or proceed on resuspend in 200 - 400 µl CHIP lysis buffer , add an equal volume of glass beads shake for 30 min at 4 °C on vortexer (maximum

DNA Isolation from Tails

Jacks Lab,Center for cancer research,MIT 1.Each tail should be in a clean eppendorf tube. 2.Add 500µl of tail lysis buffer containing Proteinase K (PK)to each tube. 3.Incubate tail samples in 5060℃water bath overnight. 4.Add 250µl saturated (6M