Annexin V-EGFP/PI Apoptosis De

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Annexin V-EGFP/PI 细胞凋亡检测试剂盒

MCE 国际站：Annexin V-EGFP/PI 细胞凋亡检测试剂盒

Annexin V-EGFP/PI 细胞凋亡检测试剂盒可方便、快捷的检测细胞凋亡。经 Annexin V-EGFP 和 PI 联合染色后，正常细胞基本无荧光（Annexin V-/PI-），早期凋亡细胞呈绿色荧光（Annexin V+/PI-），晚期凋亡细胞及坏死细胞则呈绿色和红色荧光（Annexin V+/PI+）。与 FITC 相比，EGFP 的绿色荧光更强，不易淬灭，稳定性更高。

Description & Advantages:

MCE Annexin V-EGFP/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS translocates from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca²⁺ - dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with green fluorescent EGFP can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium Iodide (PI) is a cell-membrane impermeable dye to live cells and early apoptosis cells, but stains late apoptosis cells and necrosis cells with red fluorescence. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).