HPK1-IN-14,2734168-45-5

类器官培养与应用——肺类器官

。 14B、随后将新的类器官转移至 1.5ml 离心管中，高速（约 6000g）离心 3-5s，或低速（约 300g）离心 3min。离心后，上皮碎片将沉降到底部，Matrigel 继续悬浮在培养基中，细胞团沉积在离心管底部。 15B、在显微镜下用移液器取出培养基和 Matrigel，向沉淀的细胞团内添加 200μl 新鲜的 Matrigel，混合均匀后在 24 孔板的每个培养孔中心接种 25μl 混合液。待 Matrigel 在 37℃ 培养箱中静置 10min 凝固后，加入 0.5ml 人芽尖

Lipoprotein Isolation - First week

, refractometry and dialysis.   Day 3 Lyophilization.   Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean

Lipoprotein Isolation - First week

3 Lyophilization.   Day 1: Bleeding and centrifugation. Note: You need to wear gloves throughout the procedure. Each groups takes 10-15 locusts. 1. Take 2-5 ml of PBS in a clean 100 ml beaker and add 25 µl of 200 mM PMSF