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文献和实验5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside, 0.1 M NaPO4 [pH 7] and 1 mM MgSO4 in 1.6% agar) and incubated at 30oC for up to 2 days. Transformants carrying productive lacZ fusions are recovered from the regrown SC-leu plate . It is advisable
Synthesis of Cyclopropane-Containing Leu-Enkephalin Analogs
of this chapter is to describe the preparation of a novel cyclopropane-containing cis -substituted (-Glyψ[CHOH-cp-CONH]-) subunit, which replaces Gly 2 -Gly 3 subunit of the Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) framework shown in Fig. 1 . Fig
Methods for use with the mTn-lacZ/LEU2-mutagenized library
. It is then transformed into a leu2 strain of yeast. This procedure is outlined in this figure . Use of a circle-zero strain will prevent recovery of insertions in the 2-micron plasmid. The best strategy is to screen a few thousand transformants from each pool
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