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文献和实验SH2 Domain-Based Tyrosine Phosphorylation Array
-throughput assay to detect interactions between phosphorylated proteins and human SH2 domains, in order to profile the genome-wide phosphorylation status of cells under a variety of biological conditions. The SH2 domain array was prepared by immobilizing 115
BIAcore Analysis to Test Phosphopeptide-SH2 Domain Interactions
messages, has been an enormous breakthrough (1 ). Among these transducing domains, the src homology 2 (SH2) domains are specialized in the interaction with the phosphorylated forms of tyrosine residues (2 ). SH2 domains are classically 100 amino acids
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional
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