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文献和实验Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
reporter genes can be used to measure transcription activity, such as chloramphenicol acetyl transferase (CAT), luciferase, (β-galactosidase, and human growth hormone (7 ). To examine the transcription enhancement activity of a putative promoter/enhancer
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
concentration and percentage of receptor molecules bound to the ligand in a typical amide proton exchange experiment. To ensure that 100% of receptor molecules are bound, ligand‐to‐receptor ratios > 1:1 are required when K d ≥ 10 nM. Theoretical curves for K d
Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
between promoter elements and cellular or viral regulatory factors (5 –9 ). The conventional strategy has been to transfect host cells transiently with a plasmid bearing the sequences of interest linked to a chloramphenicol acetyl transferase (CAT) reporter gene
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