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文献和实验Purification of Rat Pancreatic -Cells by Fluorescence-Activated Cell Sorting
The β-cell is receptive to intricate hormonal, neuronal and nutrient signaling which is key for normal physiology but complicates the study of specific effects of individual factors on β-cell function. To preserve the microenvironment
This chapter describes a method to measure the viability of isolated intact islets of Langerhans from rat pancreas and considers the use of isolated islets and of pancreatic beta-cell lines to study cell viability following culture. The islet
Culture of Human Pancreatic Islet Cells
, which are mainly located in the center of the islet, are the insulin-producing �;β-cells In the periphery, α-cells and δ-cells synthesizing glucagon and somatostatin, respectively, are found. Finally, a small number of cells containing pancreatic polypeptide (PP
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