Alpha MinimumEssential medium+ 2 mM Lglutamine+1.5 g/L sodium bicarbonate+ 0.2 mM inositol + 0.1 mM 2-mercaptoethanol; 0.02 mM folic acid; 100200 U/ml recombinant IL2; adjust to a final concentration of 12.5% horse serum and 12.5% fetal bovine serum