pGreenPuro shRNA expression le

Conditional Gene Expression and Knockdown Using Lentivirus Vectors Encoding shRNA

. We and others have developed a lentivector-based, conditional gene expression system for drug-controllable expression of transgenes and small hairpin RNAs (shRNAs). This system is highly robust and versatile, governing tightly controlled expression of transgenes and endogenous

增进RNAi分析的强大工具

位点提供有效的重组 BLOCK-iT™ Lentiviral RNAi Expression System 在分裂和不分裂哺乳动物细胞或者动物模型中均可获得稳定的shRNA表达 ·长期、有效的RNAi分析－有效的慢病毒整合·简单的U6框重组－att位点使得从U6入门载体的转移快速而简单·有效的慢病毒转导－为动物模型、难于转染、原代和不分裂细胞类型提供可重复的结果

v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析

a PGK or EF1a promoter driving dsRed (pBARVRand pfuBARVR) was constructed for visual detection of cellscontaining the reporter independent of reporter activation(Fig. 8.2 ). It should be noted that although the Wnt/β-catenin reportersappear