Cas9 SmartNuclease AAVS1 Posit

张峰手稿：基因工程技术之CRISPR-Cas9

cation of positive clones. Sequence the clones with a forward primer that binds the humanU6 promoter to verify the successful insertion of guide sequences. The verifi ed clones can be then prepared using a Midi or Maxiplasmid extraction kit for downstream

【求助】PKC活性 ELISA试剂盒

licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard

Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

with the reagent. As a positive control, use cells set aside at the last step of the reprogramming procedure.        b. Carry out a reverse transcription reaction using 1 µg of RNA (from step 1, above) and the SuperScript® VILO™ cDNA Synthesis Kit following