Minicircle Dual Reporter: UBC-

Two‐Hybrid Dual Bait System

for screening of these dual baits possess four separate reporter genes. A plasmid expressing an activation domain?fused protein (prey), which can be either a defined protein interactor or a cDNA library, is also expressed to allow dual hybrid?mediated

Attachment of Reporter and Conjugate Groups to the 3′ Termini of Oligonucleotides

of oligonucleotides with amino groups and attachment of amino specific reporter groups. In Protocols for Oligonucleotide Conjugates, Vol. 26: Synthesis and Analytical Techniques, pp. 93‐120. Humana Press, Totowa, N.J.

【求助】报告载体与转染

效率？ zhujoker 使用GFP做转染效率对照 woxingwosu 估计是dual luciferase reporter assay,使用两种互不干扰的luciferase：firefly luciferase用来检测激活的情况，renilla luciferase 用来作为control。 可以参考以下资料，有比较详细的介绍。 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验