Gene Knock-Out HR Targeting Ve

Receptor Knock-Out and Gene Targeting: Generation of Knock-Out Mice

Until recently, opioid receptors were studied only by a pharmacological approach because agonists and antagonists were the only tools available (1 ). Interpretation of the experimental data was complicated because of the poor selectivity

Purification of Gene Targeting Vector DNA for Electroporation

1. Purify plasmid from bacteria. We recommend the Qiagen EndoFree Plasmid Maxi kit for the purification of the targeting vector plasmid from bacteria. Please follow the directions in the kit. Electroporation of Qiagen purified DNA has been used

Targeting Vector Construction Through Recombineering

Gene targeting in mouse embryonic stem cells is an essential, yet still very expensive and highly time-consuming, tool and method to study gene function at the organismal level or to create mouse models of human diseases. Conventional cloning