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- 详细信息
- 文献和实验
- 技术资料
- 库存:
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- 英文名:
ATCC 10106
- 保质期:
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- 供应商:
武汉华尔纳生物科技有限公司
- 保存条件:
2-8度
| 出品公司: | ATCC |
| 菌种名称: | ATCC10106, ATCC 10106产黄青霉;产黄青霉菌;青霉产黄;黄青霉;菌产黄青霉Penicillium chrysogenum |
| 菌种又名: | NRRL 807 [26, CBS 306.48, IMI 24314, LSHB Ad3 LSHB P19, QM 7500] |
| 菌株类型: | 产黄青霉;产黄青霉菌;青霉产黄;黄青霉;菌产黄青霉Penicillium chrysogenum |
| 存储人: | NRRL |
| 分离来源: | 奶酪,康涅狄格州 |
| 产品目录号: | 10106 |
| 其他保藏库编号: | BCRC30564,ATCC 10106 ;CBS 306.48 ;IMI 24314 ;LSHB P.19 ;NRRL 807 ;QM 7500 |
| 培养基: | ATCC® Medium 325: Malt extract agar (Blakeslee's formula) |
| ATCC® Medium 336: Potato dextrose agar (PDA) | |
| ATCC® Medium 28: Emmons' modification of Sabouraud's agar | |
| 生长条件: | 25 ℃, 有氧 |
| 生物安全等级: | 1 |
| 模式菌株: | 是 |
| 应用: | 科研,生产,主要用于产生黄原素,质量控制应变 |
| 菌株特点: | ATCC 10106是Neurospora crassa,粗糙脉胞霉 。Nucleotide (GenBank) : U57321 Penicillium chrysogenum class I chitin synthase (CHS1) gene, |
| Nucleotide (GenBank) : U57322 Penicillium chrysogenum class II chitin synthase (CHS2) gene, | |
| Nucleotide (GenBank) : U57323 Penicillium chrysogenum class II chitin synthase (CHS3) gene, | |
| Nucleotide (GenBank) : AF173559 Penicillium chrysogenum class III chitin synthase (CHS4) gene, | |
| Nucleotide (GenBank) : AF241466 Penicillium chrysogenum small subunit ribosomal RNA gene, partial | |
| Nucleotide (GenBank) : HQ026745 ITS including 5.8S rRNA gene | |
| 参考文献: | Clutterbuck PW, et al. Studies in the biochemistry of micro-organisms. The formation from glucose by members of the Penicillium chrysogenum series of a pigment, an alkali-soluble protein and penicillin -- the antibacterial substance of Fleming. Biochem. J. 26: 1907-1918, 1932. |
| Thom C. Cultural studies of species of Penicillium. U.S. Dep. Agric. Bur. Anim. Ind. 118: 1-109, 1910. | |
| FF MicroPlate. Biolog. | |
| Walker DM, et al. New molecular markers for fungal phylogenetics: two genes for species-level systematics in the Sordariomycetes (Ascomycota). Mol Phylogenet Evol 64: 500-512, 2012. PubMed: 22626621 | |
| Ropars J, et al. A taxonomic and ecological overview of cheese fungi. Int J Food Microbiol 155: 199-210, 2012. PubMed: 22381457 | |
| Houbraken J, et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia 29: 78-100, 2012. PubMed: 23606767 | |
| Houbraken J, Frisvad JC, Samson RA. Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens. IMA Fungus 2: 87-95, 2011. PubMed: 22679592 | |
| Houbraken J, Samson RA. Phylogeny of Penicillium and the segregation of Trichocomaceae into three Families. Stud Mycol 70: 1-51, 2011. PubMed: 22308045 | |
| Peterson SW, Horn BW. Penicillium parvulum and Penicillium georgiense, sp. nov., isolated from the conidial heads of Aspergillus species. Mycologia 101: 71-83, 2009. PubMed: 19274850 | |
| An KD, et al. The identity of Penicillium sp. 1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium pineum. Antonie van Leeuwenhoek 96: 579-592, 2009. PubMed: 19784794 | |
| Wang L, Zhang XM, Zhuang WY. Penicillium macrosclerotiorum, a new species producing large sclerotia discovered in south China. Mycol Res 111: 1242-1248, 2007. PubMed: 17998158 | |
| Rakeman JL, et al. Multilocus DNA sequence comparisons rapidly identify pathogenic molds. J Clin Microbiol 43: 3324-3333, 2005. PubMed: 16000456 | |
| Wang L, et al. Penicillium persicinum, a new griseofulvin, chrysogine and roquefortine C producing species from Qinghai Province, China. Antonie van Leeuwenhoek 86: 173-179, 2004. PubMed: 15280651 | |
| Scott J, et al. Genotypic variation in Penicillium chysogenum from indoor environments. Mycologia 96: 1095-1105, 2004. PubMed: 21148929 | |
| Hong SG, et al. Sequence comparison of mitochondrial small subunit ribosomal DNA in Penicillium. J Microbiol 38: 62-65, 2000. |
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文献和实验青霉菌属于丛梗孢科。菌丝体由多数具有横隔的菌丝所组成,通常以产生分生孢子进行繁殖,产生孢子时,菌丝体顶端产生多细胞的分生孢子梗,梗的顶端分枝2—3次,每枝的末端细胞分裂成串的分生孢子,形成扫帚状。分生孢子一般呈蓝绿色,成熟后随风飞散,遇适宜环境,萌发成菌丝。青霉菌的种类很多,通常生于柑桔类水果上。蔬菜、粮食、肉类、皮革和食物上也常有分布。如产黄青霉Penicillium chrysogenum Thom、特异青霉 P. notatum Westling均能产生青霉素。黄绿青霉 P
上,经培养后,就会长出一条菌带,并产生某种抗生素向菌带周围扩散。再与这条菌带相垂直划直线接种某些不同的试验菌,就会产生不同长度的抑菌带,如图Ⅸ-3。根据抑菌带的长短,即可判断该抗生素对不同微生物的影响。本实验说明产黄青霉产生的青霉素对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑制效果。 二、实验试剂 豆芽汁葡萄糖琼脂培养基 实验设备 无菌平皿,接种环等 实验材料 产黄青霉(Peenicillium chrysogenum)、大肠杆菌、金黄
形成菌苔后,再用接种环从产黄青霉菌的菌带边缘但不要接触菌苔分别垂直向外划直线(图Ⅸ-3,2)接种大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌。 5.倒置于37℃温室培养24小时。 6.观察结果。 五、实验报告 1.结果 绘图表示并说明产黄青霉所产生的青霉素对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的抑菌效能。 2.思考题 (1)在测定抗生素的抗菌范围时,应选用什么样的试验菌
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