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- 详细信息
- 文献和实验
- 技术资料
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- 英文名:
Ethanol Standard Solution (0.3 g/L)
- CAS号:
64--17-5
- 保质期:
1年
- 供应商:
上海经科化学科技有限公司
- 保存条件:
2-8℃
- 规格:
50mL
RECOMMENDED APPLICATIONS:
Analytical standard solution for use as a control sample or calibrator with analytical test kits that measure Ethanol. Especially for use to generate calibration curves for auto-analyser or microplate assay formats with the following enzymatic tests kits: K-ETOH, K-ETOHLQR.
| CAS Number: | 64--17-5 |
| Synonyms: | Ethyl alcohol |
| Molecular Formula: | C2H5OH |
| Molecular Weight: | 46.07 |
| Concentration: | 0.3 g/L in 0.02% sodium azide |
| Content: | 50 mL |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Physical Form: | Solution |
| Stability: | > 5 years under recommended storage conditions |
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
in 9cm petri w/ 20ml aleurone solution: 10mM CaCl2 (1.47g/l) 1X antibiotic solution 100X antibiotic stock: 5mg/ml ampicillin 10mg/ml neomycin 1mg/ml nystatin GA 7mg/1ml EtOH, warm at 37C to dissolve, use 20ul/20ml aleurone
Stewart Laboratory Standard Operating Procedure
x g, 1700 rpm, 5 min in 15 ml tubes - no faster!), and resuspend in 10 mL 70% cold EtOH. Store at -20°C overnight or for several days Day 2 Pellet cells and wash once with wash buffer (PBS + 0.5% IFS). Pellet and resuspend in 0.5 mL 2M HCl
Standard hybridization technique
sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results is a variation on that described by Church and Gilbert










