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- 2025年12月11日
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- 详细信息
- 文献和实验
- 技术资料
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- 英文名：
Citric Acid Standard Solution (3 g/L)
- CAS号：
77-92-9
- 保质期：
1年
- 供应商：
上海经科化学科技有限公司
- 保存条件：
2-8℃
- 规格：
50mL
RECOMMENDED APPLICATIONS:
Analytical standard solution for use as a control sample or calibrator with analytical test kits that measure citric acid. Especially for use to generate calibration curves for auto-analyser or microplate assay formats with the following enzymatic tests kits: K-CITR.
| CAS Number: | 77-92-9 |
| Synonyms: | 2-Hydroxypropane-1,2,3-tricarboxylic acid |
| Molecular Formula: | C6H8O7 |
| Molecular Weight: | 192.12 |
| Concentration: | 3 g/L in 0.02% sodium azide |
| Content: | 50 mL |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Physical Form: | Solution |
| Stability: | > 5 years under recommended storage conditions |
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文献和实验的起始物质。因为此循环周转的强度依赖于其所含物质的量，所以必须补给转入各合成途径的物质的消耗，丙酮酸羧化酶或乙醛酸循环与此途径有关。柠檬酸循环局限在线粒体内的可溶部分（基质matrix）。循环由以下八个步骤组成：（1）草酰乙酸与乙酰CoA的缩合→柠檬酸，（2）柠檬酸的脱水加水→异柠檬酸，（3）异柠檬酸的氧化脱羧→α－酮戊二酸，（4）α－酮戊二酸的氧化脱羧与CoA的参入→琥珀酰CoA，（5）琥珀酰CoA与Pi，GDP的反应→琥珀酸、GTP、CoA，（6）琥珀酸脱氢→延胡索酸，（7）延胡索酸加水→L－
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Standard hybridization technique
sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results is a variation on that described by Church and Gilbert
