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Active Motif公司是一家致力于研发和生产核细胞分子生物学和表观遗传学研究用试剂的专业公司,也是世界著名的转录因子试剂生产商和CHIP-seq外包服务供应商。
Active Motif的产品涉及:表观遗传学相关抗体、Chip级别抗体、重组识别子和催化酶、预甲基化修饰的重组组蛋白、多聚核小体和生物素标记核小体;以及各类染色质分析、转录因子表达调控分析和DNA甲基化分析的试剂盒等。
产品详情欢迎点击链接进入官方网站查询。
https://www.activemotif.com/catalog/900/lightswitch-assay-system
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文献和实验Transient Transfection and Adrenergic Receptor Promoter Analysis
well for transfection of β 1 -AR gene fragments into adherent cell lines, but is much less effective for transfection of DNA into primary cultures. As noted by other investigators ( 5 ), we have applied FuGENE™ 6 transfection reagent, a nonliposomal lipid compound
are transfected with the 5' biotin linked sense (control) or antisense (treatment) RNAs (70-100nM final concentration (Ting et al. 2005; Kim, Villeneuve et al. 2006)) targeted to the particular promoter of choice with Lipofectamine 2000™ (Invitrogen) at a 1
Determining Relative Transduction of Adherent Cells
实验原理 MISSION® TurboGFP™ control particles enable fast determination of the appropriate transduction conditions for efficient transduction of cells with lentiviral particles. Note: The CMV promoter is known to function
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