周年庆感恩买三送一(即日起至2022.12.31)
目录号:E096-01B
产品描述:
本制品是采用SYBR Green嵌合荧光法进行Real Time PCR的专用试剂。制品中已经将DNA聚合酶、反应用Buffer、dNTPs、SYBR Green等试剂预混在一起,是一种2×浓度的预混型试剂,进行实验时,PCR反应液的配制十分方便简单。
本制品采用新型抗体修饰HotStart Taq DNA聚合酶并结合特别配方的反应缓冲液,能有效抑制非特异性反应,提高了反应特异性,能够在更广的范围内进行准确定量。
本制品配有不同浓度的ROX Reference Dye,用于不同仪器校正孔间荧光信号误差。
产品特点:
高特异性:采用新型抗体修饰的HotStart Taq DNA聚合酶,无需长时间热启动反应,大大提高了反应的高特异性。
高效:Novoprotein精心配制的Real Time PCR专用2×SuperMix,具有更高扩增效率和扩增灵敏度。
快捷:PCR反应所必需试剂集于一管之中,数分钟即可完成反应体系配制。
使用建议:
保存:原则上-20℃遮光保存。如果持续使用,可在4℃内进行短期保存,但此时须遮光,建议使用遮光箱或用添附的铝袋进行保存。
使用:请上下颠倒轻轻均匀混合,避免起泡,并经轻微离心后使用。反应液的配制、分装请使用新的(无污染的)枪头、Microtube等,避免污染。建议反应体积为20~50μl。
引物设计:一般为了增加敏感度及扩增效率且抑制非特异性反应,扩增目标序列越短越好。建议设计成200bp以下。另外对GC cluster(特别是3´末端)等,会引起引物二聚体等非特异反应,一般不建议采用,特别是Intercalater Assay受非特异性反应影响较大,更需注意。
保存温度:
-20℃
参考文献:
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For research use only.