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- Megazyme
- E-ISAMYHP
- 爱尔兰
- 2025年12月14日
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- 英文名：
Isoamylase HP
- 保质期：
2年
- 供应商：
上海经科化学科技有限公司
- 保存条件：
2-8℃
- 规格：
200 Units
High purity Isoamylase HP (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Suitable for use in ISO Standard 6647-1:2015.
| Content: | 200 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 6 months under recommended storage conditions |
| Enzyme Activity: | Isoamylase |
| EC Number: | 3.2.1.68 |
| CAZy Family: | GH13 |
| CAS Number: | 9067-73-6 |
| Synonyms: | isoamylase; glycogen 6-alpha-D-glucanohydrolase |
| Source: | Pseudomonas sp. |
| Molecular Weight: | 71,500 |
| Concentration: | Supplied at ~ 500 U/mL |
| Expression: | Purified from Pseudomonas sp. |
| Specificity: | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
| Specific Activity: | ~ 240 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg) |
| Unit Definition: | One unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC. |
| Temperature Optima: | 50oC |
| pH Optima: | 4 |
| Application examples: | Applications in carbohydrate research and in the food and feeds, and cereals industry. |
| Method recognition: | ISO Standard 6647-1:2015 |
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- 作者
- 内容
- 询问日期
文献和实验1.Characterization of the Glucan-Branching Enzyme GlgB Gene from Swine Intestinal Bacteria.
Shao, Y., Wang, W., Hu, Y. & Gänzle, M. G. (2023). Molecules, 28(4), 1881.
2.A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation.
Zhao, X., Andersson, M. & Andersson, R. (2020). Carbohydrate Polymers, 117503.
3.Structural characterization of mixed-linkage α-glucans produced by mutants of Lactobacillus reuteri TMW 1.106 dextransucrase.
Münkel, F., Fischer, A., & Wefers, D. (2020). Carbohydrate Polymers, 231, 115697.
