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- 英文名：
Oligo-α-1,6-Glucosidase plus β-Galactosidase
- 保质期：
2年
- 供应商：
上海经科化学科技有限公司
- 保存条件：
2-8℃
- 规格：
6,000 Units
| Enzyme Activity: | Oligo-1,6-α-Glucosidase, β-Galactosidase |
| EC Number: | oligo-α-1,6-glucosidase: 3.2.1.10 β-galactosidase: 3.2.1.23 |
| CAZy Family: | GH13, GH35 |
| CAS Number: | oligo-α-1,6-glucosidase: 9032-15-9 β-galactosidase: 9013-11-2 |
| Synonyms: | oligo-α-1,6-glucosidase: oligosaccharide 6-alpha-glucohydrolase β-galactosidase: beta-D-galactoside galactohydrolase |
| Source: | oligo-α-1,6-glucosidase: Microbial β-galactosidase: Aspergillus niger |
| Molecular Weight: | oligo-α-1,6-glucosidase: 67,300 β-galactosidase: 125,000 |
| Concentration: | Oligo-α-1,6-glucosidase (2,000 U/mL) / β-galactosidase (2,000 U/mL) |
| Expression: | Oligo-α-1,6-glucosidase: Recombinant from a Microbial source β-Glucosidase: Purified from Aspergillus niger |
| Specificity: | This enzyme mixture gives complete hydrolysis of isomaltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf. |
| Specific Activity: | Oligo-α-1,6-glucosidase (~ 170 U/mg) / β-galactosidase (~ 170 U/mg) |
| Unit Definition: | Oligo-α-1,6-glucosidase: One Unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40oC. β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC. |
| Temperature Optima: | 50oC |
| pH Optima: | 4.5 |
| Application examples: | Used for removal of isomaltose and lactose in the measurement of dietary fiber. |
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, with an acidic pH optimum (4 ) and a neutral, magnesiumdependent (5 -7 ) enzyme; β-glucosidase and α- or β-galactosidase (8 –11 ); N -acetyl hexosaminidase (12 –14 ); neuraminidase (15 ); phospholipase A1 (16 ,17 ), lysophospholipase (18 –20
