Devoid of α-amylase, β-glucanase (cellulase), endo-1,4-β-xylanase and polygalacturonanase (determined by incubating high levels of the enzyme with pure polysaccharides in highly sensitive viscometric assays).
Specific Activity:
~ 9 U/mg (40oC, pH 7.0 on N, N-dimethylcasein)
Unit Definition:
One Unit of activity is the amount of enzyme that will produce one µmole (181 µg) of tyrosine equivalents from N, N-dimethylcasein per minute at pH 7.0 and 40oC (colour determined using the Folin-Ciocalteu reagent).