目录号:E043-01B
产品描述:
第一链cDNA合成预混液是以mRNA或者总RNA为模板,高效合成第一链cDNA。本产品含有反转录反应所需的全部试剂(NovoScript® Reverse Transcriptase,RNase Inhibitor,Oligo(dT)18,Random N6,dNTPs),浓度为2×。反应时只需要加入RNA模板和水即可,操作简单,减少操作过程中的污染。合成的第一链cDNA可直接用于PCR或荧光定量PCR的模板,第二链cDNA的合成或线性RNA扩增,也可用于需要用带有放射性或非放射性核苷酸标记第一链cDNA的实验。
产品特点:
本制品中使用的NovoScript Reverse Transcriptase无RNase H活性,可避免第一链cDNA合成过程中,RNA/DNA杂交模板链中RNA降解,保证了cDNA合成质量。
本制品中Random N6和Oligo(dT)18引物比例经过优化,可均匀合成样品中的cDNA。
操作简单,只需要加入RNA模板和水即可完成反应体系配制,降低了操作过程中的污染机会。
保存温度:
-20℃
注意事项:
1)用DEPC处理实验用到的所有实验器材,或购买已经证明无核酸酶的实验用具,实验过程戴手套并经常更换手套,避免RNA酶的污染。
2)确保所用试剂中无RNA酶污染。
3)试剂盒要严格密封保存。在反转录过程中,所有管子确保扣严。
4)纯化过的RNA必须保证不含有盐,金属离子,乙醇和苯酚,以上成分会干扰第一链cDNA合成反应。可采用乙醇沉淀RNA的方法去除掉痕量污染物。
5)为保证反转录反应有效进行,需使用高质量的RNA模板。
6)有实验结果提示,本反转录酶可在15分钟内完成1kb反转录反应。
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For research use only.