#周年庆感恩买三送一(即日起至2022.12.31)
产品描述 (Description):
第一链cDNA合成预混液是以mRNA或者总RNA为模板,高效合成第一链cDNA。本产品含有反转录反应所需的全部试剂(NovoScript® II Reverse Transcriptase,RNase Inhibitor,Oligo(dT)18,Random N6,dNTPs)。反应时只需要加入RNA模板和水即可,操作简单,降低操作过程中的污染。本制品含有去基因组成分gDNA Purge,以RNA为模板进行cDNA第一链合成时,可以同步去除基因组DNA污染,反应结束后,75℃加热5分钟,就可以失活反转录酶,RNA酶抑制剂,gDNA Purge。合成的第一链cDNA能直接用于PCR或荧光定量PCR的模板,第二链cDNA的合成或线性RNA扩增,也可用于需要用带有放射性或非放射性核苷酸标记第一链cDNA的实验。
产品特点 (Features):
本制品采用耐高温逆转录酶,大幅度提高了热稳定性和cDNA合成效率。
本制品中添加的NovoScript Reverse Transcriptase无RNase H活性,可避免第一链cDNA合成过程中,RNA/DNA杂交模板链中RNA降解,保证cDNA合成的质量。
本制品中Random N6和Oligo(dT)18引物比例经过优化,可均匀合成样品中的cDNA。
本制品中含有去基因组成分,进一步提高定量准确性。
产品组成 (Components):
产品组成 |
A包装 |
B包装 |
2×NovoScript® Plus 1st Strand cDNA Synthesis SuperMix |
500μl |
1ml |
gDNA Purge |
50μl |
100μl |
RNase Free Water |
1ml |
1ml×2 |
保存温度(Temperature):
-20℃
注意事项(Notice):
1)用DEPC处理实验用到的所有实验器材,或者购买已经证明无核酸酶的实验用具,实验过程戴手套并经常更换手套,避免RNA酶的污染。
2)确保所用试剂中无RNA酶污染。
3)试剂盒要严格密封保存。在反转录过程中,所有管子要确保扣严。
4)纯化过的RNA必须保证不含有盐,金属离子,乙醇和苯酚,以上成分会干扰第一链cDNA的合成反应。可采用乙醇沉淀RNA的方法去除掉痕量污染物。
5)为保证反转录反应的有效进行,需使用高质量的RNA模板。
6)有实验结果提示,本反转录酶可在20分钟内完成11kb反转录反应。
参考文献(References):
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Chronic oral administration of P. gingivalis induces microglial activation and degeneration of dopaminergic neurons possibly through increase in gut permeability and peripheral IL-17A in LRRK2 R1441G mice[J]. Yu-kun Feng. et al. Journal of Neuroinflammation. 2020.
Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization[J]. Bing Dong. et al. Stem Cell Research & Therapy. 2021.
COL1A1 as a potential new biomarker and therapeutic target for type 2 diabetes[J]. Guopeng Lin. et al. Pharmacological Research. 2021.
Oral P. gingivalis impairs gut permeability and mediates immune responses associated with neurodegeneration in LRRK2 R1441G mice[J]. Yu-kun Feng. et al. Journal of Neuroinflammation. 2021.
Combined cytotoxicity of polystyrene nanoplastics and phthalate esters on human lung epithelial A549 cells and its mechanism[J]. Qingying Shi. et al. Ecotoxicology and Environmental Safety. 2021.
Anti-inflammatory effects of Platycodin D on dextran sulfate sodium (DSS) induced colitis and E. coli Lipopolysaccharide (LPS) induced inflammation[J]. Ruiqi Guo. et al. International Immunopharmacology. 2021.
Inhibition of FvMYB10 transcriptional activity promotes color loss in strawberry fruit[J]. Guanqun Chen. et al. Plant Science. 2020.
A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair[J]. Zhengwei Deng. et al. Experimental Cell Research. 2020.
A poplar B-box protein PtrBBX23 modulates the accumulation of anthocyanins and proanthocyanidins in response to high light[J]. Chaofeng Li et al. Plant Cell And Environment. 2021.
For research use only.