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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
8. Analyze the stained cells by flow cytometry using violet (~405 nm) excitation and violet fluorescence emission (~450 nm) for the calcein violet (live cells) and blue-green fluorescence emission (~525 nm) for the aqua-fluorescent reactive dye
Cell Counting/ Live-Dead Discrimination
when you calculate original numbers Green is Live , Red is Dead Note: these chemicals are highly carcinogenic. PI can be used in place of EB at the same concentration
Differential Staining of Live and Dead Embryos using Fluorescein Diacetate
for accumulation). 6) View embryos or oocytes for staining using the fluorescence microscope under UV epiluminesence (use UV filter). Live Stain=Green Dead Stain=Red/Orange
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