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- Invitrogen-Dynal
- 2026年01月14日
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文献和实验Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer
2. To the chilled microfuge tube, add: 0.1 M dithiothreitol 2.5 μl placental RNase inhibitor 200 units oligo(dT)12-18 10 μl 10x reverse transcriptase buffer 25 μl 20 mM solution of dGTP, dATP, and dTTP 10 μl 125 μM dCTP 10 μl 10 mCi/ml
. 2. Preparation of Dynabeads® Oligo (dT)25 1) Resuspend Dynabeads® Oligo(dT)25 thoroughly before use. 2) Transfer the desired volume of beads from the stock tube to a RNase-free 1.5 ml microcentrifuge tube and place
for large-scale purification (>25 µg) of poly(A)+ RNAextracted from mammalian cells. Typically, between 1% and 10% of the RNA applied to the oligo(dT) column is recovered as poly(A)+ RNA. Because the method can be frustratingly slow, it is not recommended
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