Anti-GAPDH Monoclonal antibody

产品简介

英文名称	Anti-GAPDH Monoclonal antibody
中文名称	3-磷酸甘油醛脱氢酶抗体
别名	GAPDH
背景资料	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
Swiss Prot	P04406
Gene ID	2597
亚细胞定位	Cytoskeleton Cytosol Nucleus
克隆类型	Monoclonal Antibody
亚型	IgG1
蛋白分子量	36kDa
稀释比例	WB 1:3000-8000. IHC 1:100-300. IF 1:100-200.
免疫原	Recombinant protein of human GAPDH
Public Immunogen Range	Recombinant protein of human GAPDH
纯化方法	Affinity purification
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存	Store at -20°C. Avoid freeze / thaw cycles.
性状	液体
交叉反应	Human Dog Rabbit Pig Mouse Rat
应用	WB IF IHC
规格	50ul 100ul
单位	支

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with GAPDH antibody diluted at 1:3000;Lane: HeLa