- ￥1600
- Solarbio
- 中国 北京
- K200097M-100ul
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京索莱宝科技有限公司
- 规格：
100ul
Anti-SARS-CoV-2 Nucleocapsid Monoclonal Antibody
产品简介
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Western blot analysis with SARS-CoV-2 Nucleocapsid antibody diluted at 1:5000.
产品简介
| 英文名称 | Anti-SARS-CoV-2 Nucleocapsid Monoclonal Antibody |
| 靶标 | SARS-CoV-2 Nucleocapsid |
| 别名 | 2019-nCoV Nucleocapsid;SARS-CoV-2 Nucleocapsid Protein;SARS-CoV-2 NP;2019-nCoV N |
| 宿主 | Mouse |
| 稀释比例 | WB 1:5000-8000. ELISA 1:5000-50000. |
| Gene ID | 43740575 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| Swiss Prot | P0DTC9 |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 蛋白分子量 | 46kDa |
| 免疫原 | Recombinant Protein |
| 亚型 | IgG1 |
| 纯化方法 | Affinity purification |
| 克隆号 | 6G5 |
| 背景资料 | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 克隆类型 | Monoclonal Antibody |
| 交叉反应 | SARS-CoV-2 |
| 应用 | WB Elisa |
| 规格 | 50ul 100ul |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Western blot analysis with SARS-CoV-2 Nucleocapsid antibody diluted at 1:5000.
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文献和实验相关实验
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
a simple ELISA method to detect human anti-antibody response.
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Anti-SARS-CoV-2 Nucleocapsid Monoclonal Antibody
￥1600
