Anti-HSPA5 Monoclonal Antibody

产品简介

单位	支
英文名称	Anti-HSPA5 Monoclonal Antibody
别名	GRP-78;BiP;HSP70 family protein 5;
宿主	Mouse
稀释比例	WB 1:1000-3000. IHC 1:100-300.
Gene ID	3309
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P11021
蛋白分子量	72kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm
免疫原	Synthetic Peptide
亚型	IgG1
纯化方法	Affinity purification
克隆号	8G3
背景资料	Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) . In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.
克隆类型	Monoclonal Antibody
交叉反应	Human Pig Rat
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Immunohistochemistry of paraffin-embedded Human stomach cancer with HSPA5 antibody diluted at 1:200

Immunohistochemistry of paraffin-embedded Human liver cancer with HSPA5 antibody diluted at 1:200

Immunohistochemistry of paraffin-embedded Human colorectal cancer with HSPA5 antibody diluted at 1:200

Immunohistochemistry of paraffin-embedded Human breast cancer with HSPA5 antibody diluted at 1:200

Western blot analysis with HSPA5 antibody diluted at 1:2000;Lane:Hela