Anti-HSPA8 Monoclonal Antibody

产品简介

单位	支
英文名称	Anti-HSPA8 Monoclonal Antibody
别名	LAP-1;LPS-associated protein 1;HSC70;HSP73;HSPA10
宿主	Mouse
稀释比例	WB 1:2000-5000. IHC 1:50-200.
Gene ID	3312
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P11142
蛋白分子量	71/54kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm Nucleus
免疫原	Recombinant Protein
亚型	IgG1
纯化方法	Affinity purification
克隆号	6C7
背景资料	Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. HSP70 goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The HSP70-associated co-chaperones are of three types: J-domain co-chaperones HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase STUB1. Interacts with VGF-derived peptide TLQP-21.
克隆类型	Monoclonal Antibody
交叉反应	Human Rat Mouse
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Immunohistochemistry of paraffin-embedded Human testis cancer with HSPA8 antibody diluted at 1:100

Immunohistochemistry of paraffin-embedded Human prostate cancer with HSPA8 antibody diluted at 1:100

Immunohistochemistry of paraffin-embedded Human colorectal cancer with HSPA8 antibody diluted at 1:100

Western blot analysis with HSPA8 antibody diluted at 1:3000;Lane:Mouse brain