Anti-AAK1 Polyclonal Antibody

Anti-AAK1 Polyclonal Antibody



产品简介

靶标	AAK1
别名	AAK 1; Aak1; AAK1_HUMAN; Adaptor associated kinase 1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2 associated protein kinase 1; AP2-associated protein kinase 1
宿主	Rabbit
稀释比例	WB 1:1000-3000. IHC 1:50-200.
Gene ID	22848
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q2M2I8
蛋白分子量	94/104kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cell membrane Cytoplasm
免疫原	A synthetic peptide of human AAK1
亚型	IgG
纯化方法	Affinity purification
背景资料	Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:17494869, PubMed:11877457, PubMed:11877461, PubMed:12952931, PubMed:14617351, PubMed:25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed:17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed:11877457, PubMed:18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed:12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed:18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed:21464124).
Public Immunogen Range	670-720/961aa
克隆类型	Polyclonal Antibody
交叉反应	Human Predicted:Cow Pig
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Western blot analysis with AAK1 antibody diluted at 1:2000;Lane:M��

Immunohistochemistry of paraffin-embedded Human prostate cancer with AAK1 antibody diluted at 1:100.