Anti-SIAH2 Polyclonal Antibody

Anti-SIAH2 Polyclonal Antibody


产品简介

英文名称	Anti-SIAH2 Polyclonal Antibody
靶标	SIAH2
别名	E3 ubiquitin-protein ligase SIAH2 ;RING-type E3 ubiquitin transferase SIAH2;Seven in absentia homolog 2;Siah-2;hSiah2;
宿主	Rabbit
稀释比例	IHC 1:50-200.
Gene ID	6478
保存	Store at -39°C. Avoid freeze / thaw cycles.
Swiss Prot	O43255
蛋白分子量	35kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.22.
亚细胞定位	Nucleus Cytoplasm
免疫原	A synthetic peptide of human SIAH2
亚型	IgG
纯化方法	Affinity purification
背景资料	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:9334332, PubMed:11483518, PubMed:19224863). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:9334332, PubMed:11483518, PubMed:19224863). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PubMed:9334332, PubMed:11483518, PubMed:19224863). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) (PubMed:9334332, PubMed:11483518, PubMed:19224863). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia (PubMed:22878263). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes (PubMed:9334332, PubMed:11483518, PubMed:19224863, PubMed:22878263). Has some overlapping function with SIAH1 (PubMed:9334332, PubMed:11483518, PubMed:19224863). Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not (PubMed:12411493). Promotes monoubiquitination of SNCA (PubMed:19224863). Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation (PubMed:26392558). Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PubMed:26392558). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 (By similarity).
Public Immunogen Range	105-155/324
克隆类型	Polyclonal Antibody
交叉反应	Human Predicted:Zebrafish Xenopus laevis Rat Mouse
应用	IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Immunohistochemistry of paraffin-embedded Human breast cancer with SIAH2 antibody diluted at 1:100

Immunohistochemistry of paraffin-embedded Human stomach cancer with SIAH2 antibody diluted at 1:100