Anti-WDR61 Polyclonal Antibody

Anti-WDR61 Polyclonal Antibody


产品简介

靶标	WDR61
别名	WDR61;REC14;SKI8
宿主	Rabbit
稀释比例	IHC 1:100-300.
Gene ID	80349
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q9GZS3
蛋白分子量	34kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm Nucleus
免疫原	A synthetic peptide of human WDR61
亚型	IgG
纯化方法	Affinity purification
背景资料	Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
Public Immunogen Range	90-140/305
克隆类型	Polyclonal Antibody
交叉反应	Human Predicted:Cow Mouse
应用	IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Immunohistochemistry of paraffin-embedded Human thyroid cancer using WDR61 antibody diluted at 1:200.