Anti-PRKCI Polyclonal Antibody

Anti-PRKCI Polyclonal Antibody



产品简介

靶标	PRKCI
别名	PKCI;DXS1179E;nPKC-iota;Protein kinase C iota type;Atypical protein kinase C-lambda/iota;PRKC-lambda/iota;aPKC-lambda/iota;
宿主	Rabbit
稀释比例	IHC 1:100-300.
Gene ID	5584
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	P41743
蛋白分子量	68kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cell membrane Cytoplasm Nucleus
免疫原	Recombinant protein of human PRKCI
亚型	IgG
纯化方法	Affinity purification
背景资料	Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation (By similarity).
Public Immunogen Range	1-350aa
克隆类型	Polyclonal Antibody
交叉反应	Human Rat Mouse
应用	IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Immunohistochemistry of paraffin-embedded Human colorectal cancer using PRKCI antibody diluted at 1:200