AP endonuclease XTH2;APEX nuclease 2;APEX nuclease-like 2;AP endonuclease 2
宿主
Rabbit
稀释比例
WB 1:1000-3000.
Gene ID
27301
保存
Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot
Q9UBZ4
蛋白分子量
57kDa
储存液
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位
Nucleus Cytoplasm
免疫原
Recombinant protein of human APEX2
亚型
IgG
纯化方法
Octanoic acid-ammonium sulfate precipitation
背景资料
Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
Public Immunogen Range
172-521aa
克隆类型
Polyclonal Antibody
交叉反应
Human Rat Mouse
应用
WB
规格
50ul 100ul
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Western blot analysis with APEX2 antibody diluted at 1:2000;Lane:U87,PC-3M-IE8,U937,Raw264.7