Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位
Cytoplasm Cell membrane
免疫原
Recombinant protein of human RAB3A
亚型
IgG
纯化方法
Affinity purification
背景资料
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT.
Public Immunogen Range
1-220aa
克隆类型
Polyclonal Antibody
交叉反应
Human Rat Mouse
应用
IHC
规格
50ul 100ul
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Immunohistochemistry of paraffin-embedded Rat brain with RAB3A antibody diluted at 1:200