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- Invitrogen-CABU
- 2025年12月29日
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文献和实验. 7.TRITC,10% on celite (Research Organics). 8.Bio-Beads SM-2 in 0.7x15 column. Procedure (perform under reduced light) 1.Get 1-2 mg vinculin,stored in 2 mM Pipes,0.02% Azide,pH 7.6,in liquid nitrogen.Thaw quickly in warm water and chill in ice. 2.Mix
.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation
2 mg TRITC, incubate 30 minutes at 37 ° C on a rotary shaker. Wash 2 x in Na2 HPO4 (50 mM, pH 9.2) and 4 x in PB. Freeze aliquots of 109 particles/ml at -20 ° C. Phagocytosis assay
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